高原平原人群基因多态性及低氧相关基因的研究.docVIP

高原平原人群基因多态性及低氧相关基因的研究 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：高原平原人群基因多态性及低氧相关基因的研究 1 1 资料与方法 3 2 结果 4 文2：高原平原人群基因多态性及低氧相关基因的研究 5 1 资料与方法 6 2 结果 8 3 讨论 9 参考文摘引言： 13 原创性声明（模板） 13 文章致谢（模板） 14 正文 高原平原人群基因多态性及低氧相关基因的研究 文1：高原平原人群基因多态性及低氧相关基因的研究 Study of gene polymorphism and hypoxia related gene on residents livin at high altitude and plain area 【Abstract】 Objective This paper researched angiotein converting enzyme（ACE） gene polymorphism of Tibetan，Mongolian，Han Chinese residents living at high altitude and Han Chinese living at plain area.Methods Applying the technology of polymeae chain reaction（PCR） gene amplification and polyacrylamide gel（PAG） electric charge movement as well as gene sequencing，aims to undetand the relatiohip between ACE gene frequency，ACE gene type frequency and ACE gene character with hypoxia inducible factor gene（HIFG）.Results Gene frequency is 0.6562，0.6377，0.6505 and 0.7186 in Han Chinese group，Tibetan group，Mongolian group at high altitude and Han Chinese at plain area respectively；there are not significant differences among three high altitude groups，but there is significant difference respectively compare to plain area（P＜0.001）.The gene type frequency is I/I 14%，I/D 11%，D/D 4% in Htan Chinese group at high altitude；I/I 16%，I/D 10%，D/D 7% in Tibetan group，I/I 14%，I/D 12%，D/D 6% in Mongolian group and I/I 5%，I/D 3%，D/D 26% in Han Chinese group at plain area；the result shows that there is minor difference among three high altitude groups，but there is significant difference respectively compare to plain are data（P＜0.001）.In addition，there is gene expression at 490bp point of gene sequence on Wistar rat.Conclusion This study provides molecular bioloic basis for further studying genetic mechanism of hypoxia adoptation of human beings at high altitude. 【Key words】 Tibetan；mongolian；Han Chinese；gene polymorphism；hypoxia 自然界的生物有多种多样，但每种生物的个体之间又存在着许多差异，一种生物群体尽管在整体水平上有它本身的相同的生物特性，可是在整体某一性状方面存在着个体差异，这种形状的差异科学家称为多态性现象（Polym