黄连素和牛磺酸对胰岛素抵抗大鼠脂肪组织PAIlmRNA表达的影响（临床医学范文）.docVIP

黄连素和牛磺酸对胰岛素抵抗大鼠脂肪组织PAIlmRNA表达的影响（临床医学范文） 目录 TOC \o 1-9 \h \z \u 目录 1 正文 2 文1：黄连素和牛磺酸对胰岛素抵抗大鼠脂肪组织PAIlmRNA表达的影响 2 1 资料与方法 3 1.1 动物 3 1.2 药物与制备 3 1.3 主要试剂与仪器 4 1.4 造模方法及实验分组 4 1.5 观测指标 4 1.6 RTPCR方法 4 1.6.1 总RNA提取 5 1.6.2 逆转录及PCR反应 5 1.6.3 2％琼脂糖凝胶电泳 5 1.6.4 扫描成像分析 6 1.7 统计学方法 6 文2：黄连素和牛磺酸对胰岛素抵抗大鼠脂肪组织PAIlmRNA表达的影响 6 1 资料与方法 7 1.1 动物 7 1.2 药物与制备 8 1.3 主要试剂与仪器 8 1.4 造模方法及实验分组 8 1.5 观测指标 8 1.6 RTPCR方法 9 2％琼脂糖凝胶电泳 10 1.7 统计学方法 10 2 结　　果 10 3 讨　　论 11 参考文摘引言： 13 原创性声明（模板） 15 文章致谢（模板） 15 正文 黄连素和牛磺酸对胰岛素抵抗大鼠脂肪组织PAIlmRNA表达的影响（临床医学范文） 文1：黄连素和牛磺酸对胰岛素抵抗大鼠脂肪组织PAIlmRNA表达的影响 Abstract Objective：To observe the effects of berberine and taurine on PAI1mRNA expression in epiploon adipose tissue of high fat rats model of iulin resistance(IR).Methods：SD rats were divided into normal and model groups at random by weight.Model groups were fed with high fat diet for 30 days fitly，screened the rats whose ISI decreased as model rats and treated with berberine and taurine respectively and combinedly for 30 dyas.After treatment，using RTPCR to PAI1mRNA from adipose tissue，the products were obtained，once its seqence was verified，semiquantitative RTPCR employed to study its expressio in adipose tissues of rats with different groups.The fasting plasma glucose，iulin，TG，TC and HDLC were performed in each groups.ISI was calculated.Results：The IR model rats were induced by high fat diet.The berberine and taurine group and combine group could significantly increase iulin seitivity (P＜0.05)，decrease the weight(P＜0.05)，and decrease the expression of PAI1mRNA (P＜0.05) in model rats.Meanwhile TG，TC，LDLC decreased and HDLC increased.The paramete mentioned above in combine group was better than other groups.Conclusion：Application of combining berberine and taurine is effective in increasing iulin seitivity IR in rats induced by highfat diet and decreasing the expression of PAI1mRNA in adipose tissue