趋化因子CCL5在类风湿关节炎患者滑液炎性细胞中的表达.docVIP

趋化因子CCL5在类风湿关节炎患者滑液炎性细胞中的表达 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：趋化因子CCL5在类风湿关节炎患者滑液炎性细胞中的表达 1 1 资料与方法 3 2 结果 4 文2：趋化因子CCL5在类风湿关节炎患者滑液炎性细胞中的表达 5 1 资料与方法 6 2 结果 7 3 讨论 8 参考文摘引言： 10 原创性声明（模板） 11 文章致谢（模板） 12 正文 趋化因子CCL5在类风湿关节炎患者滑液炎性细胞中的表达 文1：趋化因子CCL5在类风湿关节炎患者滑液炎性细胞中的表达 Production of CC chemokine CCL5 in synovial fluid inflammatory cells from patients with rheumatoid arthritis 【Abstract】 Objective To determine the CC chemokine CCL5 (RANTES) level in serum and synovial fluid (SF) from patients with rheumatoid arthritis (RA)，and the expression of CCL5 in synovial fluid inflammatory cells.Methods CCL5 in serum and SF was quantified by ELISA in 28 RA patients and 21 OA patients. The production of CCL5 by SF inflammatory cells in vitro was also determined ，and compared with that by peripheral white blood cells.Results Serum CCL5 level in RA was higher than that in OA， moreover the concentration of CCL5 in SF was greater than in serum in RA. Serum CCL5 level correlated significantly with joint swelling index， ESR and CRP. Higher production of CCL5 was found in SF inflammatory cells from RA as compared with SF inflammatory cells from OA， and peripheral white blood cells from RA.Conclusion The increased level of CCL5 in RA， as well as the correlation of serum CCL5 with clinical and laboratory paramete of RA， indicate that CCL5 play an important role in RA. The increased production CCL5 by SF inflammatory cells suggests that those cells are main source of CCL5 secretion in RA. 【Key words】 arthritis rheumatoid chemokine CCL5 类风湿关节炎（RA）是一种常见的慢性炎性关节炎，其病理特点为大量炎性细胞在滑膜的浸润和在滑液的渗出，及由此导致滑膜增生、血管翳形成，最终造成关节软骨破坏。近期的研究显示，趋化因子（chemokine）在RA的炎性细胞关节浸润中起着极为重要的作用［1］。现已发现CC亚族趋化因子受体5（CCR5）在RA关节浸润的炎性细胞中表达增高［2］，提示RA患者病变关节内CCR5的配体产生增多，选择性地趋化CCR5阳性白细胞亚群进入炎性关节部位。本文通过采用酶联免疫吸附法(ELISA)测定RA患者血液和滑液中CCR5配体CCL5 （又称调节活化的正常T细胞表达和分泌的因子(Regulated on Activation Normal T Cell Expressed and Secreted，RANTES)）水平，并对RA患者外周血白细胞、滑液炎性细胞产生CCL5进行体外研究，以探讨CCL5与RA的相关性，及其在RA患者的产生来源。 1 资料与方法 1.1 一般