甲氨喋呤和雷公藤对类风湿关节炎滑膜细胞产生趋化因子的影响.docVIP

甲氨喋呤和雷公藤对类风湿关节炎滑膜细胞产生趋化因子的影响 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：甲氨喋呤和雷公藤对类风湿关节炎滑膜细胞产生趋化因子的影响 1 1 资料与方法 3 2 结果 4 文2：甲氨喋呤和雷公藤对类风湿关节炎滑膜细胞产生趋化因子的影响 4 1 资料与方法 5 3 讨论 7 参考文摘引言： 9 原创性声明（模板） 11 文章致谢（模板） 11 正文 甲氨喋呤和雷公藤对类风湿关节炎滑膜细胞产生趋化因子的影响 文1：甲氨喋呤和雷公藤对类风湿关节炎滑膜细胞产生趋化因子的影响 The inhibitory effects of methotrexate and tripterygium glycosides on chemokine CCL5 production in synoviocytes from patients with rheumatoid arthritis 【Abstract】 Objective To determine the inhibitory effects of methotrexate (MTX) and tripterygium glycosides (TG) on chemokine CCL5 production in synoviocytes isolated from patients with rheumatoid arthritis (RA).Methods Synoviocytes， isolated from RA patients and patients with osteoarthritis (OA)， were cultured and stimulated with or without lipopolysaccharide (LPS) in the presence or absence of different dosage of MTX or TG. CCL5 level in cultural supernatants was measured by enzyme-linked immunosorbent assay (ELISA).Results RA synoviocytes spontaneously produced higher level of CCL5 than OA synoviocytes did. Both MTX and TG were found to significantly inhibit LPS-induced CCL5 production in RA synoviocytes. Moreover， the inhibitory effect of MTX appeared to be in a dosage dependent manner in the concentration ranged from 0- 20ng/ml. Whereas TG showed its inhibitory effect only when it reached at the concentration of 60ng/ml. Furthermore， the inhibitory effect of TG was found to be correlated with TG-induced cell death.Conclusion The increased production of CCL5 by RA synoviocytes indicates a role of CCL5 in the pathogenesis of RA， and the disease modifying effects of MTX and TG might be existed through their anti-CCL5 effect. 【Key words】 arthritis rheumatoid chemokines CC CCL5 类风湿关节炎（RA）是一种常见的慢性炎性关节炎，其病理特征为大量炎性细胞在滑膜的浸润和在滑液的渗出，及由此导致的滑膜增生，血管翳形成，最终造成关节软骨破坏。近期的研究结果显示，趋化因子（chemokine）在RA的炎性细胞关节浸润中起着极为重要的作用［1］。我们发现RA患者血清和滑液CC趋化因子受体5（CCR5）的配体CCL5（RANTES）水平增高，并证实滑膜细胞是其产生的主要部位，并且在用甲氨喋呤（MTX）和雷公藤多甙（TG）治疗后，CCL5水平下降［2，3］。为了进一步探讨MTX和TG在RA滑膜