孕激素和米非司酮对子宫内膜癌HHUA细胞的作用.docVIP

孕激素和米非司酮对子宫内膜癌HHUA细胞的作用 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：孕激素和米非司酮对子宫内膜癌HHUA细胞的作用 1 1 材料与方法 3 1.4 四甲基偶氮唑盐（MTT）比色实验 4 1.5 免疫组织化学法 5 2 结果 6 文2：孕激素和米非司酮对子宫内膜癌HHUA细胞的作用 6 1 材料与方法 8 1.4 四甲基偶氮唑盐（MTT）比色实验 9 1.5 免疫组织化学法 10 2 结果 11 3 讨论 12 参考文摘引言： 14 原创性声明（模板） 15 文章致谢（模板） 16 正文 孕激素和米非司酮对子宫内膜癌HHUA细胞的作用 文1：孕激素和米非司酮对子宫内膜癌HHUA细胞的作用 Effects of progesterone and mifepristone on the human endometrial cancer cell line HHUA 【Abstract】 Objective To study the effects of progesterone and mifepristone on the growth inhibitio and apoptosis protein of human endometrial cancer cell line.Methods HHUA cells，treated by different concentratio of progesterone and mifepristone were examined for their growth inhibitio with methyl thiazolyl tetrazolium（MTT） assay after 72 hou and got the inhibitory concentration 50%（IC50）. HHUA cells，treated by control，progesterone or mifepristone or progesterone combined mifepristone for 48 hou，were examined for their inhibitory rate with MTT and for their bcl-2 and Fas with immunohistochemistry assay.Results HHUA cells growth was inhibited by progesterone and mifepristone.IC50 of progesterone is 100.7537μmol/L；IC50 of mifepristone is 39.5534μmol/L.The inhibitory rate of progesterone combined with mifepristone was obviously stronger than that of progesterone and mifepristone. The expression of bcl-2 was decreased and Fas increased（P＜0.05）. Conclusion HHUA cells was treated by progesterone combined with mifepristone and synergistic effect was observed on the inhibitory growth and the expression of bcl-2 and Fas. 【Key words】 endometrial neoplasms；mifepristone；progesterone；tumor cells；culture 近20余年来，子宫内膜癌的发病率有上升趋势，且随着发病年龄的年轻化，保留生育能力的治疗方案已成为患者的迫切需求。激素治疗是年轻子宫内膜癌患者的主要保守治疗手段之一，研究显示［1］，年轻未育的内膜癌Ⅰ期患者，如果细胞分化好可以考虑保守治疗，同时严密监测有无复发，等待完成生育后再行手术治疗。目前子宫内膜癌激素治疗的主要药物有高效孕激素及三苯氧胺。有研究表明，孕激素受体拮抗剂米非司酮对多种肿瘤细胞具有抑制作用。本实验旨在探索孕激素和米非司酮二者联合对子宫内膜癌HHUA细胞的生长抑制作用，以及该方案对HHUA细胞株表达凋亡蛋白的影响，以期为临床治疗子宫内膜癌提供新方案。 1 材料与方法 1.1 子宫内膜癌细胞系 子宫内膜癌HHUA细胞是高分化子宫内膜腺癌细胞，其