BSJ-01-175-DataSheet-生命科学试剂-MedChemExpress.pdf

Hotline: 400-820-3792 Inhibitors • Screening Libraries • Proteins www.MedChemE BSJ-01-175 Cat. No.: HY-145072 CAS No.: 2227392-55-2 分⼦式: C₃₀H₃₃ClN₆O₂ 分⼦量: 545.08 作⽤靶点: CDK 作⽤通路: Cell Cycle/DNA Damage 储存⽅式: Please store the product under the recommended conditions in the Certificate of Analysis. BIOLOGICAL ACTIVITY ⽣物活性 BSJ-01-175 是⼀种有效的、选择性 CDK12/13 共价抑制剂。BSJ-01-175 表现出对癌细胞卓越的选择性、对磷 酸化RNA 聚合酶 II 的有效抑制以及显著下调 CDK12 靶向基因 [1]。 IC50 Target CDK12 CDK13 体外研究 BSJ-01–175 (0-10 μM; 72 hours) causes a 5-fold increase in cell viability compared to the wild type (WT), indicating strong dependence on covalent bond formation with Cys1039 [1]. BSJ-01–175 (0-10 μM; 72 hours) slightly decreases the activity of TC71 Ewing sarcoma cells compared to THZ531 [1]. BSJ-01–175 (0-5 μM) specifically targets CDK12/13 and suppresses the transcription of BRAC1 and BRAC2 [1]. Cell Viability Assay [1] Cell Line: Kelly wild type or CDK12C1039F cells Concentration: 0-10 μM Incubation Time: 72 hours Result: Observed a 5-fold increase in cell viability compared to the wild type (WT), indicating