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会计学1InhibitionofExperimentalCornealNeovascularizationby实验性角膜新生血管的抑制作用
Purpose To evaluate and compare the inhibitory effects of topical high dose, low-dose and subconjunctival bevacizumab (Avastin, Genentech Inc., San Francisco, Ca, USA) on corneal vascularization in a rat model 第1页/共12页
MethodsCorneas of 20 rats (Sprague-Dawley, male) were chemically cauterized with silver nitrate sticks in order to induce neovascularizationAnimals were divided in four groups Group 1: Control group that received only topical artificial tear twice dailyGrup 2: Subconjunctival injection group that received 0.05 ml (1.25mg) of bevacizumab on day 1, 4 and 7Group 3: Low-dose topical bevacizumab (4 mg/ml) group that received twice daily Group 4: High-dose topical bevacizumab (12.5 mg/ml) group that received twice daily第2页/共12页
MethodsDigital photographs of the corneas were taken and analyzed using an image analysis software (Pixcavator Image Analyzer, Intelligent Perception,WV, USA) On day 10, all animals were sacrificed and the eyes were enucleated Corneas were excised and examined histopathologically 第3页/共12页
Control group Group 2 Group 3Group 4Mean percentage of neovascularized corneal area (%)63.32± 13.10 30.22 ± 15.73 26.76± 10.23 25.52 ± 12.45 p value 0.01 0.01 0.01 Results第4页/共12页
Control groupTopical high-dose bevacizumab group (12.5 mg/ml) 第5页/共12页
Control groupTopical low-dose bevacizumab group (4 mg/ml) 第6页/共12页
Subconjunctival bevacizumab group /0,05ml) Control group第7页/共12页
ResultsIn histological examination of the excised corneas, treated eyes showed significantly less neovascular areas and number of vessels in group 2,3 and 4 than the control groupThe differences between control group and treatment groups were found to be statistically significant (p 5 for all) Bevacizumab is able to inhibit corneal angiogenesis, without any difference of this effect with Changing the route of administration (subconjunctival or topical) Increasing the dosage (4mg/ml or 12.5mg
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