miR在豆科植物苜蓿根中对其菌根化和分生组织活性的影响.pptxVIP

会计学1miR在豆科植物苜蓿根中对其菌根化和分生组织活性的影响 2023/1/82主要内容INTRODUCTIONRESULTS 1、 Mtr-miR396a and mtr-miR396b genes show different tissue-specific expression profiles in Medicago truncatula roots 2、In M. truncatula roots, miR396 regulates six GRF and two bHLH79 transcription factors 3、Characterization of the miR396 regulatory network in M. truncatula第1页/共36页 4、 miR396 negatively affects root growth, RAM size and cell proliferation in the root apex 5、 Silencing of MtGRF genes mimics the miR396-OE root growth phenotype 6、 Hormonal control of miR396 expression 7、miR396 limits mycorrhizal colonization but not nodulation2023/1/83第2页/共36页 INTRODUCTIONIn plants, root growth is controlled via coordinated cell division and expansion at the root apex, where three regions are observed: (i) the meristematic zone, (ii) the elongation zone, and (iii) the differentiation zoneRoot development is regulated by complex interactions of hormone signalling pathways2023/1/84第3页/共36页 In recent years, microRNAs (miRNAs) have emerged as important regulators of the architecture of the root systemin Arabidopsis thaliana, miR165/166 governs the radial patterning of vascular and pericycle tissues via quantitative repression of HD-ZIP III transcription factorsA set of auxin-related miRNAs, including miR160, also regulate lateral root initiation and/or root growth2023/1/85第4页/共36页 However, few miRNAs have been reported to play roles in RAM functionAlthough large sets of mycorrhization or nodulation-responsive miRNAs have been identified in legumes, few have been functionally linked to these processes.2023/1/86第5页/共36页 In A. thaliana, miR396 is encoded by two loci, post-transcriptionally represses several members of the growth-regulating factor (GRF) family. These plant-specific TFs control the growth and development of leaves and stems.miR396 may have evolved to play roles in various regulatory networks in plants.In this paper, we describe the roles of the miR396 regulatory network in M. t