半干旱雨养区苜蓿种植年限影响黄绵土N2O排放的微生物驱动机制.pdf

10.96%-31.58% 、10.95%-23.07%和 2.56%-15.96% ，随苜蓿种植年限的延长 而 增 加 ， 亚 硝 化 螺 菌 属 相 对 丰 度 显 著 增 加 ， norank_p__ammonia_oxidising_bacteria_ensembl 相对丰度显著降低。 （5 ）RDA 分析结果表明，土壤有机碳（P=0.004 ）、硝态氮（P=0.025 ）、微生物 量碳（P=0.013 ）、微生物量氮（P=0.048 ）和脲酶（P=0.005 ）是影响AOA 群落 结构的主要因素；土壤 NH4+-N （P=0.014 ）和微生物量碳（P=0.037 ）是影响AOB 群落结构的主要因素。 （6 ）N O 排放通量同时受到环境因素和生物因素的共同影响，其中土壤温度、 2 SOC、TN、NO --N、MBC 和 pH 是影响半干旱黄绵土区苜蓿土壤 N O 排放通量 3 2 的环境因素； AOA 群落的 norank_c__environmental_samples_p__Crenarchaeota 、 unclassified_k__norank_d__Archaea 、 norank_c__environmental_samples_p__Thaumarchaeota 和 norank_p__environmental_samples_k__norank 及 AOB 群 落 的 unclassified_k__norank_d__Bacteria 影响黄绵土 N O 排放通量的主要生物因子。 2 关键字：黄绵土，紫花苜蓿，N O 排放通量；氨氧化古菌；氨氧化细菌； 2 Summary Alfalfa plays an important role in environment construction and industrial structure adjustment because of its excellent feeding value and ecological function in Loess Plateau. In view of the study on microbiological mechanism of N O emission of 2 alfalfa in loessial soil is less, so, this study took the alfalfa with different growth years in semi-arid rain-fed agricultural area in Loess Plateau as research object, static chamber-gas chromatographic techniques were used to continuously measure and analyze the N O fluxes of alfalfa with different growth year(L2003,L2005,L2012) 2 during 2018 to 2019, quantitative PCR and Illumina MiSeq high-throughput sequencing were applied to investigate abundance, community structure, and diversity of ammonia- o