生物分离工程双语版.pptVIP

IV high-resolution fractionation Previous purification steps (1) volume reduction (2) clarification (3) adjustment of crude extract Chromatography steps (1) cation exchange (2) anion exchange (3) affinity chromatography (4) hydrophobic chromatography 第六十二页，共七十二页。 A chromatography procedures Affinity chromatography (1) nonspecific adsorption (2) expensive labile ligand (3) difficulties of adsorbent sterilization (4) biomimetic ligand (5) natural ligand expensive and decreased activity when immobilized. (6) pseudo-ligand (7) ligand leakage and product contamination Large scale pur