基因概念的发展.pptxVIP

第八章 基因概念的开展;一、基因和DNA 1、测序： 〔1〕、化学降解法 美国A.Maxam 和W.Gilbert创造;标记; T C A G;〔2〕双脱氧终止法〔链终止法〕 〔英〕F.Sanger 和A.X .Coulson创造;DNA sequencing using the chain termination method. ;DNA sequencing gel showing the separation of fragments in the four sequencing reactions (one per lane). ;In DNA sequencing using dideoxynucleotides labeled with fluorescent dyes, all four ddNTPs are added to the same tube, and during primer extension, all combinations of chains are produced. ;2、隔裂基因和重叠基因 　　;二、生化突变型与一基因一酶学说;Beadle and Tatum: 一个基因 --- 一种酶 One Gene --- One En