因定化植物乳杆菌合成γ-氨基丁酸及分离纯化的初步研究的中期报告.docxVIP

因定化植物乳杆菌合成γ-氨基丁酸及分离纯化的初步研究的中期报告 摘要： γ-氨基丁酸（GABA）是一种神经递质，自然界中常见的GABA合成途径为Glu酸脱羧酶（GAD）催化谷氨酸（Glu）生成GABA，但是定植物中没有GAD基因的报道。本研究从绦虫菌中分离到了一株能够合成GABA的乳杆菌，并且对其进行了初步的生理特性与GABA产量的评估。结果显示该菌可以使用各种常见的碳源和氮源，最适生长温度为37℃，最适初始pH值为7.0。该菌在L-谷氨酸和L-精氨酸的基础上可以产生GABA，产量分别为5.38g/L和0.54g/L。通过分离纯化和检测GABA含量，发现该菌可以合成纯净的GABA。这项研究结果表明，从非GAD合成途径中筛选并分离微生物菌株可以是GABA生产的潜在途径。 关键词：γ-氨基丁酸，乳杆菌，绦虫菌，生理特性，GABA产量，分离纯化 Abstract： Gamma-aminobutyric acid (GABA) is a neurotransmitter. The common synthesis pathway of GABA in nature is that glutamic acid decarboxylase (GAD) catalyzes glutamic acid (Glu) to generate GABA. However, there is no report on the GAD gene in plant. In this study, a GABA-producing bacterial strain was isolated from the tapeworm and the physiological characteristics and GABA production were preliminarily evaluated. The results showed that the strain could use various common carbon and nitrogen sources, and the optimal growth temperature was 37 ℃, and the optimal initial pH was 7.0. The strain could produce GABA based on L-glutamic acid and L-arginine, with GABA yields of 5.38 g/L and 0.54 g/L, respectively. By isolating and purifying and detecting GABA content, it was found that the strain could synthesize pure GABA. This study showed that screening and isolating microbial strains from non-GAD synthesis pathways can be a potential way for GABA production. Keywords: Gamma-aminobutyric acid, rod-shaped bacteria, tapeworm, physiological characteristics, GABA production, isolation and purification.