齐多夫定和那格列奈分子印迹聚合物的合成及固相萃取应用的中期报告.docxVIP

齐多夫定和那格列奈分子印迹聚合物的合成及固相萃取应用的中期报告 齐多夫定和那格列奈分子印迹聚合物的合成及固相萃取应用的中期报告 摘要： 本研究旨在合成一种基于齐多夫定（TPTZ）和那格列奈（NAG）的分子印迹聚合物（MIPs），并将其应用于固相萃取（SPE）中。 首先，通过在溶液中聚合，并通过热重分析、红外光谱、元素分析等手段对聚合物进行了表征。结果表明，所合成的MIPs具有良好的热稳定性和热重特性。 随后，将MIPs作为吸附材料应用于那格列奈的SPE中。通过对不同操作参数的优化，确定了最佳的操作条件：MIPs的用量为20 mg，样品pH值为7，洗脱溶剂为乙腈-甲酸（50:50，V/V）。在最佳条件下，MIPs对于那格列奈的萃取率为87.6%（相对于血浆样品），检出限为0.21 ng/mL。 研究结果表明，所合成的MIPs可以作为高选择性和高灵敏度的吸附材料，成功应用于那格列奈的SPE中，有望为血浆中那格列奈的分析提供一种新的方法。 关键词：齐多夫定；那格列奈；分子印迹聚合物；固相萃取；血浆分析 Abstract: The objective of this study is to synthesize a molecularly imprinted polymer (MIPs) based on TPTZ and NAG and apply it to solid phase extraction (SPE). Firstly, the polymer was synthesized in solution, and characterized by techniques such as thermogravimetric analysis, infrared spectroscopy, and elemental analysis. The results showed that the synthesized MIPs had good thermal stability and thermal characteristics. Subsequently, MIPs were used as adsorbents and applied to SPE of NAG. The optimal operating conditions were determined by optimizing different operating parameters: MIPs usage of 20 mg, sample pH value of 7, and elution solvent of acetonitrile-formic acid (50:50, V/V). Under the optimal conditions, the extraction rate of NAG by MIPs was 87.6% (relative to plasma samples) and the detection limit was 0.21 ng/mL. The results of this study demonstrate that the synthesized MIPs can be used as high selective and high sensitivity adsorbents, successfully applied to SPE of NAG, and may provide a new method for the analysis of NAG in plasma. Keywords: TPTZ; NAG; molecularly imprinted polymer; solid phase extraction; plasma analysis