拟南芥微丝结合蛋白ADF7对根毛发育的影响.docxVIP

拟南芥微丝结合蛋白ADF7对根毛发育的影响 摘要　　根毛是植物体重要的结构，参与植物的多种生命活动，对植物的生长发育至关重要，所以对根毛生长发育的研究显得尤为重要。根毛发育也是根毛形成过程，由根表皮细胞的2类细胞决定， H细胞和N细胞。微丝骨架是细胞内支撑细胞形态的重要细胞器，在根毛发育中起到重要调节作用。然而目前人们对根毛发育中微丝骨架的分子机制理解甚少。本实验发现了微丝解聚因子 ADF7 在根毛发育中发挥作用，并进一步探究了 ADF7 调节微丝动态参与根毛发育的过程。具体结果如下： 　　1.鉴定adf7 纯合突变体，构建和获得 ADF7 过表达和恢复突变体的纯合植株，完成了ADF7 各种遗传材料（包括adf7突变体，ADF7 过表达植株和ADF7恢复突变植株，下同）的准备。 　　2.通过显著性差异分析得出突变体材料 adf7-2 和 adf7- 3的根毛数量与野生型相比显著减少，相反，过表达材料 ADF7 OE #13和 ADF7 OE #14的根毛数量与野生型相比显著增加，而我们将突变的材料恢复，可以看到恢复突变材料 ADF7 comp #2和 ADF7 comp #7的根毛数量与野生型相比并无明显差异。 　　3.通过对 ADF7 各种遗传材料根毛数量统计发现，与野生型相比，adf7的根毛数量明显减少，ADF7过表达植株的根毛数量显著增多，而ADF7恢复突变植株的根毛数量则与野生型相近，表明 ADF7 在根毛发育中起到正向调节作用。综上，本实验证明在根毛发育过程中微丝解聚因子 ADF7发挥正向调节作用。 关键词：拟南芥，根毛，微丝解聚，ADF7 Abstract 　　 Root hair is an important structure of plant body weight, participating in a variety of plant life activities, and is very important for the growth and development of plants, so the study on the growth and development of root hair is particularly important. Root hair development is also a process of root hair formation and is determined by two types of cells of root epidermal cells, H cells and N cells. Microfilament cytoskeleton is an important organelle supporting cell morphology in cells and plays an important role in regulating root hair development. However, the molecular mechanism of the microfilament skeleton in root hair development is poorly understood. In this study, we found that the microfilament depolymerization factor ADF7 played a role in the development of root hair, and further explored the process of ADF7 regulating the dynamic involvement of microfilaments in the development of root hair. The specific results were as follows: 　　1.Homozygous ADF7 mutants were identified, homozygous ADF7 overexpressed and restored mutants were constructed and obtained, and preparations for various genetic materials of ADF7 (including ADF7 mutant, ADF7 overexpressed plant and ADF7 restored mutant plant, the same below) were completed. Significant difference analysis showed that t