藏獒源犬细小病毒四川株的分离鉴定及中药体外抗病毒效果试验的中期报告.docxVIP

藏獒源犬细小病毒四川株的分离鉴定及中药体外抗病毒效果试验的中期报告 摘要 本研究旨在分离鉴定藏獒源犬细小病毒四川株，并评估中药在体外的抗病毒效果。经PCR扩增和测序分析，成功分离到细小病毒四川株，基因序列与已知的细小病毒序列高度一致。同时，选取多种中药进行体外的抗病毒试验，结果显示，青黛（50mg/mL）、熟地黄（50mg/mL）和连翘（100mg/mL）对细小病毒四川株均具有明显的抑制作用（P0.05），其中熟地黄的抑制率最高，达到了90%。综上所述，本研究为进一步研究藏獒源犬细小病毒的生物学特性及中药的抗病毒机制提供了一定的参考。 关键词 藏獒源犬；细小病毒；中药；抗病毒 Abstract The aim of this study was to isolate and identify the Sichuan strain of the canine parvovirus from Tibetan mastiff, and to evaluate the antiviral effect of traditional Chinese medicine in vitro. After PCR amplification and sequencing analysis, the Sichuan strain of the canine parvovirus was successfully isolated, and the gene sequence was highly consistent with the known canine parvovirus sequence. At the same time, a variety of traditional Chinese medicines were selected for in vitro antiviral experiments. The results showed that indigo (50mg/mL), cooked rhubarb (50mg/mL), and forsythia (100mg/mL) had significant inhibitory effects on the Sichuan strain of canine parvovirus (P0.05), and cooked rhubarb had the highest inhibition rate, reaching 90%. In summary, this study provides a reference for further research on the biological characteristics of canine parvovirus in Tibetan mastiffs and the antiviral mechanism of traditional Chinese medicine. Keywords Tibetan mastiff; canine parvovirus; traditional Chinese medicine; antiviral