(8.9)--维持间充质干细胞干性的策略与机制.pdfVIP

维持间充质干细胞干性的策略与机制

摘要间充质干细胞(mesenchymalstemcells,MSCs)是一种具有维持自我更新能力以及多向

分化潜能的多能干细胞,同时具有低免疫原性和高便携性的特点,是组织工程理想种子细胞的重要

来源。组织工程中需要大量种子细胞,因此对MSCs进行体外扩增至关重要。MSCs的体外培养方

式多以传统二维(two-dimensional,2D)培养为主,然而在这种传统的2D培养过程中MSCs往往会出

现自分化现象,导致其失去干性。MSCs的干性可以通过生物反应器、超低吸附表面、悬滴培养或

支架培养等三维(three-dimensional,3D)培养方式进行维持。该文主要从3D培养方式出发,叙述其

维持MSCs干性的作用、优势及相关机制。

关键词间充质干细胞;干性;3D培养;2D培养

StrategiesandRegulatoryMechanismsforMaintainingtheStemness

ofMesenchymalStemCells

1,222*1*

JINGHua,LINKaili,ZHAOCancan,ZHAOBin

1

(SchoolofMaterialsandChemistry,UniversityofShanghaiforScienceandTechnology,Shanghai200093,China;

2ShanghaiNinthPeoplesHospital,ShanghaiJiaoTongUniversitySchoolofMedicine,Shanghai200011,China)

AbstractMSCs(mesenchymalstemcells)arepluripotentstemcellswiththepotentialtomaintainself-

renewalandmulti-directionaldifferentiation,andhavethecharacteristicsoflowimmunogenicityandhighportabil-

ity.Theyareanimportantsourceofidealseedcellsfortissueengineering.Alargenumberofseedcellsareneeded

intissueengineering,soitisveryimportanttoamplifyMSCsinvitro.TheinvitrocultureofMSCsismainlybased

ontraditional2D(two-dimensional)culture.However,intheprocessoftraditionaltwo-dimensionalculture,MSCs

oftenoccurself-differentiation,whichcausesthemtolosestemness.ThestemnessofMSCscanbemaintainedby

3D(three-dimensional)culturemethodssuchasbioreactor,ultra-lowadsorptionsurface,hangingdropcultureor

scaffoldculture.Thispapermainlystartsfromthe3Dculturemethod,anddescribesitsstemnessmaintenanceef-

fect,advantagesandrelatedmechanismsonMSCs.

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