免疫胶体金技术.pptVIP

  • 3
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  • 约7.73千字
  • 约 33页
  • 2024-05-15 发布于广东
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1.Pipette1mlofcolloidintoeachofaseriesof3mlcleanplastictubes.2.Adjusttheantibody(0.1ug/ul)totheproperpHwith100nMK2CO3or100mMHC1.3.Addtheantibodytoeachtubeinaseriesfrom0-150ul(ie0-15uginstepsof0,1,2,3....15ug).4.Shakeeachtubeandleaveforapproximately5minutestoconjugate.6.Toeachtubeadd100ulof10%NaC1andagitatefor1minute.7.TheTubecontainingtheminimumamountofproteinrequiredtostabilizethegoldsolisindicatedbytheoneinwhichthecolorofthegoldsoldoesnotchangefromredtoblueupontheadditiono

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