高效液相色谱仪器维护和保养.ppt

HPLC故障诊断基线不稳定。基线漂移：色谱柱未平衡好，柱温未稳定，流动相变化，等。基线噪音：可能原因是：流动池有气泡，流动池被污染，对紫外检测器可将流动池移走即可确认。色谱柱和或系统受污染。灯能量不足。外界因素影响，如电源，温度和湿度，震动，等。第31页,共32页，星期六，2024年，5月感谢大家观看第32页,共32页，星期六，2024年，5月**Pointoutthemainfeatures.Everythinghereshouldberecognizablealready!**Pointoutthemainfeatures.Everythinghereshouldberecognizablealready!**Pointoutthemainfeatures.Everythinghereshouldberecognizablealready!**Pointoutthemainfeatures.Everythinghereshouldberecognizablealready!**Pointoutthemainfeatures.Everythinghereshouldberecognizablealready!**Pointoutthemainfeatures.Everythinghereshouldberecognizablealready!**Pointoutthemainfeatures.Everythinghereshouldberecognizablealready!**Pointoutthemainfeatures.Everythinghereshouldberecognizablealready!**Pointoutthemainfeatures.Everythinghereshouldberecognizablealready!**更换非互溶性的流动相，需准备能与新旧流动相互溶的中介溶剂。**Pointoutthemainfeatures.Everythinghereshouldberecognizablealready!**Tocontinuewiththeconceptofmatchedfittings,thisslideshowsvariouscolumnconnectorsusedinHPLC.Noticethatforeachvendor,thedistancefromtheendofthetubingtowheretheferrulesareseatedorswagedisdifferent?Ifthedistanceistoogreatfortheendfittingofthecolumn,theferrulewillnotsealwiththecolumnandthefittingwillleak.Ifyouthentightendownonthefitting,thetubingwillpuncturethecolumnfrit.Ifthedistanceistooshort,youcansealtheferruleandnothavealeak,buttherewillbealargeextracolumnvolumeforbandbroadeningtooccur.Noticehowtheferrulesareshapeddifferentlyaswell?Somearedesignedtobeusedwithcolumnsandsomearespecifictoinjectorsordetectorsorpumps.DONOTINTERCHANGETHESE!Theymaybecomeapermanentfixture,orimbeddedinyourcolumnorequipment.Thelastpointis,onceyouseattheferruleo