兔肝VX2肿瘤射频消融后不同区带VEGF的表达和细胞凋亡 .pdfVIP

兔肝VX2肿瘤射频消融后不同区带VEGF的表达和细胞凋亡

刘亚;吕维富;朱先海;施长杲;王亚光;刘开才;成德雷;周春泽;鲁东

【摘要】目的分析兔肝VX2肿瘤射频消融(RFA)后不同区带血管内皮细胞生长因

子(VEGF)的表达和肿瘤细胞凋亡情况.方法将48只兔肝移植VX2肿瘤,建立动物

模型,分为实验组(n=42)和对照组(n=6).对实验组行RFA,分别在术后即刻、1天、

2天、1周、2周、3周各处死7只实验兔,留存肿瘤标本,进行HE染色、VEGF检

测、AnnexinV-FITC/PI标记和流式细胞仪检测,观察不同时间段、不同区带的

VEGF变化及细胞凋亡情况.结果实验组RFA术后针道炭化区、热凝固区和消融边

界区平均VEGF值差异均有统计学意义(P均＜0.05);针道炭化区、热凝固区及边界

区术后即刻与其他时间点间两两比较差异均有统计学意义(P均＜0.05);针道炭化区

及热凝固区VEGF在术后即刻达高峰,术后1天～3周呈整体下降趋势,消融边界区

VEGF在术后即刻到1周呈上升趋势,2周后下降.术后针道炭化区、热凝固区和消

融边界区平均肿瘤细胞凋亡率与对照组比较差异均有统计学意义(P均＜0.05),各区

带细胞凋亡率均在术后1天达高峰,后呈下降趋势.结论RFA后针道区和热凝固区

内肿瘤细胞VEGF下降和肿瘤凋亡显著,而消融交界区仍可能有存活的肿瘤细胞;在

RFA后第3周时,残留肿瘤细胞的增殖能力可恢复到术前状态,此时宜进一步采取相

应的治疗措施.%ObjectiveToexploretheexpressionofvascularendothelial

growthfactor(VEGF)andapoptosisofthetumorcellsinthedifferent

regionsofrabbitliverVX2tumorafterradiofrequencyablation

(RFA).MethodsForty-eightexperimentalrabbitswereimplantedwithVX2

tumor.Aftersuccessfullyestablishedthemodel,therabbitswererandomly

dividedintocontrolgroup(n=6)andRFAgroup(n=42).IntheRFAgroup,7

rabbitsateachtimepointwerekilledatimmediately,1day,2days,1week,2

weeks,3weeksafterRFA,andthetumorspecimenswereretainedand

performedwithHEstaining,VEGF,AnnexinV-FITC/PIlabeling,flow

cytometryanalysis.ThechangesofVEGFandapoptosisofthecellsin

differentperiodsanddifferentzonesafterRFAwereobserved.ResultsAfter

theoperation,thedifferenceoftheVEGFvalueoftheneedle

zone,coagulationnecroticzoneandjunctionzonehadstatistically

significant(allP＜0.05).ThedifferenceoftheVEGFvalueineachzone

betweenimmediatelyandtheothertimepointsafteroperationby

pairwisecomparisonsweresignificantlydifferent(allP＜0.05).TheVEGF

valueoftheneedlezoneandcoagulationnecroticzonereachedthepeak

afteroperationimmediately,whichdeclinedfrom1dayto3weeksafter

operative.AndVEGFofthe