实训项目二药品生物检定基本操作技术二PracticalTrainingProgramIIBasic.pptx

;学习目标

Learningobjective;;材料与仪器

Materialsandinstruments

菌种葡萄球菌

Strainsstaphylococcus

培养基固体培养基和液体培养基

2.CulturemediumSolidculturemediumandliquidculturemedium

3.仪器接种环、酒精灯、恒温箱、酒精棉球、打火机、记号笔、试管架等。

3.InstrumentsInoculatingrings,alcohollamps,thermostats,alcoholcottonballs,lighters,markers,testtuberacks,etc.;一、培养基及制备方法

I.Culturemediumandpreparationmethods;2．按用途划分为：

Dividedbyuse:

基础培养基：普通琼脂培养基

Basalculturemedium:commonagarculturemedium

增菌培养基：专一性强，使目的菌扩大生长。

Enrichmentculturemedium:strongspecialization,sothatthetargetbacteriatoexpandthegrowth.

鉴别培养基、选择培养基：根据各种细菌的生化特性鉴别种属的培养基

Indicatorculturemedium,selectiveculturemedium:identifytheculturemediumofspeciesaccordingtothebiochemicalcharacteristicsofvariousbacteria;第二节药品生物检定技术的基本操作

SectionIIBasicoperationofbiologicalassaytechnologyofdrugs;Preparationofculturemedium;案例

Case;案例

Case;制作平板

Makeaplate;二、微生物接种技术

Ⅱ.Microbialinoculationtechnology;2．玻璃涂棒

2.Glasscoatingbar;3．滴管或吸管

3.Dropperorpipette;（二）无菌操作

(II)Asepticoperation;接种环境

Inoculationenvironment;于接种之前打开接种室和超净工作台紫外灯消毒30min，同时打开风机；

Beforeinoculation,turnontheUVlampsintheinoculatingroomandthecleanbenchtodisinfectfor30minutes,andturnonthefanatthesametime;

接种时必须关闭紫外灯

TheUVlampsmustbeturnedoffduringinoculation;要点：防止污染，严格的无菌操作

Keypoints:preventcontamination,strictasepticoperation

接种者的双手要进行消毒处理，一般用75%酒精或0.01%新洁尔灭擦洗

Thehandsoftheinoculatorsshouldbesterilized,usuallywith75%alcoholor0.01%bromogeraminescrubbing

整个操作过程都要靠近酒精灯火焰

Thewholeoperationshouldbeclosetotheflameofthealcohollamp

接种工具、管、瓶口接种前后要通过灼烧灭菌

Inoculatingtools,tubesandvialsshouldbesterilizedbycauterizationbeforeandafterinoculation.

棉塞不得乱放，操作中只能夹在手上

Cottonplugsshouldnotbemisplacedandshouldonlybeheldinthehandduringoperation.

不能用嘴直接吸吹吸管。

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