生物化学Ⅱ复习题：糖酵解与三羧酸循环关键问题.pdfVIP

BiochemistryⅡReviewQuestions

byXuJiuyang

PartⅠGlycolysisTCACycle

1.Wheredoseglycolysis,PyruvateAcetyl-CoA,andTCAcycletakece?

2.Whatarethe2characteristicsofsuccinatedehydrogenase?

3.Arealltheintermediatesinglycolysisphosphorylated?Why?

4.Foreveryglucoseinglycolysis,howmanyATP,NADHareproducedinpreparatoryphase

andpay-offphase,respectively?

5.Whatarethe3enzymesand5cofactorsofPDH(pyruvatedehydrogenasecomplex)?

6.HowaretheelectronsreceivedbyNAD+andFADtransferredrespectively?

7.Howisthehexokinaseinliverregulated?Whataboutinskeletalmuscle?Canglycogen

phosphorylaseabeinhibitedbyglucose?How?

8.WhatisthefunctionofcofactorTPP?Whataboutbiotin,PLP(pyridoxalphosphate)and

tetrahydrofolate?

9.CanPDHberegulatedcovalentlybyphosphorylation?How?

10.What’stheproductofpentosephosphatepathway?Andwhat’sthefunctionofthepathway?

Canmlssynthesizeglucosefrompyruvate?Why?(Lackingwhichcycle?)Whatabout

fromAcetyl-CoA?

AnswersforPartⅠ

1.Glycolysis:cytosol;PDH:mitochondria(innermembrane);TCAcycle:mitochondria

2.GeneratingFADH2,boundtotheinnermembrane*(alwaysemphasizedinclass)

3.Allphosphorylated(it’stomakeyourecitethewholepathway^_^);1)preventfrombeing

exportedoutofcell;2)conservedenergy;3)higherbindingspecificity

4.Preparatoryphase:-2ATP,0NADH;Pay-offphase:4ATP,2NADH(2triose)

5.E1(TPP),E2(lipoate),E3(FAD)+NAD+CoA-SH

+--++-++-+-

6.NAD(H=2e+H),FAD(2H=2H+2e);NAD+H+2e=NADH,FAD+2H+2e=FA