响应面法优化超声波辅助提取虎奶菇多酚工艺及其抗氧化活性分析.pdfVIP

120粮食与油脂2025年第38卷第4期

响应面法优化超声波辅助提取

虎奶菇多酚工艺及其抗氧化活性分析

傅志丰，王慧宾，邓朝阳，韩晓丹，徐志勇，顾震

（江西省科学院应用化学研究所，江西南昌330096）

摘要：以虎奶菇为原料,采用超声波辅助乙醇溶液提取虎奶菇多酚。以多酚得率为指标，在单

因素试验基础上，通过响应面法优化提取工艺，并对提取物的抗氧化活性进行测定。结果表明：最

佳提取工艺为超声功率300W、提取时间56min、乙醇体积分数44%、提取温度59℃，在此工艺条

件下，多酚得率为（1.022±0.013)mg/g。抗氧化试验测定发现，虎奶菇多酚具有较好的抗氧化活

性,其清除DPPH自由基和羟基自由基的ICso分别为2.010mg/mL和0.430mg/mL。

关键词：超声波辅助提取；虎奶菇；多酚；响应面法；抗氧化活性

Optimizationofultrasoud-assistedextractionofpolyphenols

fromPleurotustuber-regium（Fr.）Sing

byresponsesurfacemethodologyandanalysisofitsantioxidantactivity

FUZhifeng,WANGHuibin,DENGChaoyang,

HANXiaodan,XUZhiyong,GUZhen

(InstituteofAppliedChemistry,JiangxiAcademyofSciences,Nanchang330096,Jiangxi,China)

Abstract:UsingPleurotustuber-regium(Fr.)Singasrawmaterial,polyphenolswereextractedbyultra-

sound-assistedethanolsolution.Takingtheyieldofpolyphenolsastheindex,theextractionprocesswas

optimizedbyresponsesurfacemethodologybasedonsinglefactorexperiments,andtheantioxidantactivi-

tyoftheextractwasdetermined.Theresultsshowedthattheoptimalextractionprocesswereultrasound

power300W,extractiontime56min,ethanolvolumefraction44%,andextractiontemperature59℃.

Undertheseprocessconditions,thepolyphenolyieldwas(1.022+0.013)mg/g.Theantioxidanttest

showedthatpolyphenolsfromPleurotustuber-regium(Fr.)Singhadgoodantioxidantactivity,andtheICso

ofthescavengingDPPHfreeradicalsandhydroxylfreeradicalswere2.010mg/mLand0.430mg/mL,

respectively.

Keywords:ultrasound-assistedextraction;Pleurotustuber-regium(Fr.)Sing;polyphenol;response

surfacemethodology;antioxidantactivity