沙蓬全基因组Survey测序分析.pdfVIP

分子植物育种，2025年，第23卷，第3期，第894-898页

MolecularPlantBr,No.3,894-898

研究报告

ResearchReport

沙蓬全基因组Survey测序分析

杨文静张丽甘晓燕聂峰杰巩刘璇宋玉霞

宁夏农林科学院农业生物技术研究中心，银川，750004

*通信作者，songyx666@163.com

摘要沙蓬作为流动沙丘上的先锋植物群落，种子蛋白质含量高，具有重要的生态经济意义，对其基因组

的研究有助于深入开展分子遗传学研究，并为绘制全基因组精细图谱提供依据。本研究运用二代高通量测序

技术，获得137.75Gb有效数据，总测序深度为89x，以K=17进行K-mer分析并修正后，拟南芥的基因组估

计为1.02Gbbp、

605.2Mb的ContigN50和3453bp、644.4Mb的ScaffoldN50

面的基因组学分析，并在此基础上对其进行深入的基因组学分析。

关键词沙蓬(Agriophyllumsquarrosum);基因组;Survey测序

GenomeSurveyAnalysisofAriophvllumsa

uarrosum

GanXiaoyan

YangWenjingZhangLiSongYuxia

NieFengjieGongLeiLiuXuan

AgriculturalBiotechnologyResearchCenterofNingxiaAcademyofAgricultureandForestrySciences,Yinchuan,750004

*Correspondingauthox666@163.com

DOI:10.13271/j.mpb.023.000894

AbstractAsapioneerplantcommunityonmovingsanddunes,theseedproteincontentofAgriophyllumsquar-

rosumishigh,whichhasimportantecologicalandeconomicsignificance.ThestudyofthegenomeofAgriophyl-

lumsquarrosumishelpfulforfurthermoleculargeneticresearchandprovidesabasisforthedetailedmappingof

thewholegenome.Inthisstudy,thesecond-generationhigh-throughputsequencingtechnologywasusedtoobtain

137.75Gbofeffectivedata,andthetotalsequencingdepthwas89x.AfterK-meranalysisatK-17andcorrection,

theestimatedgenomesizeofAgriophyllumsquarrosumwas1.02Gb,theheterozygousrateofthegenomewas

0.49%,andtheproportionofrepeatedsequenceswas70.18%,GCcontentwas38.46%,whichwasamicro-

heterozygoushigh-repeatgenome.Afterinitialassembly,ContigsequenceN50was1569bpwithatotallengthof

605.2MbandscaffoldsequenceN50was3453bpwi