医学论文：杜仲总多糖对环磷酰胺致免疫低下小鼠的影响.docVIP

??????????????? 作者：辛晓明,王浩,冯蕾,朱玉云,高允生【摘要】? 目的 研究杜仲总多糖对环磷酰胺致免疫低下小鼠的影响。方法 应用环磷酰胺建立免疫低下小鼠模型,给予不同剂量杜仲总多糖后,测定小鼠体重、胸腺指数、脾指数、腹腔巨噬细胞吞噬率、吞噬指数等指标。结果 300 g杜仲原药材经提取分离得9.3 g多糖干品。应用环磷酰胺后,小鼠体重、胸腺指数、腹腔巨噬细胞吞噬率、吞噬指数均降低。杜仲总多糖能减轻环磷酰胺致小鼠体重的下降,升高免疫低下小鼠胸腺指数,明显增加小鼠腹腔巨噬细胞吞噬率、吞噬指数(p0.01)。与空白组比较,杜仲总多糖还能提高正常小鼠的脾指数(p0.05)。结论 杜仲总多糖能够较好地增强小鼠免疫功能。 【关键词】? 杜仲总多糖；环磷酰胺；免疫调节；小鼠　　abstract：objective to study the immunoregulatory activity of eucommia ulmoides oliv polysaccharides (eop) on immunodepressive mice. method the immunodepressive mice model was established with cyclophosphamide (cy) treated. eop was given with different doses, and mouse weight, thymus index, spleen index, abdominal cavity macrophage engulfing rates, engulfing index were measured. result 300 grams eucommia ulmoides oliv crude drugs after withdraw separate gained 9.3 grams polysaccharides. after cy injection, the mouse weight, chest gland index, abdominal cavity macrophage swallowing rate, phagocytic count reduced. eop could resist the mouse body weight drop induced by cy, elevate the thymus index of immunity low mouse, obviously increase mouse abdominal cavity macrophage engulfing rates and engulfing index (p0.01). and compared with the blank group, eop could promote the spleen weight of immunity low mouse (p0.05). conclusion eop could enhance immunofunction of immunodepressive mice. 　　key words：eucommia ulmoides oliv polysaccharides；cyclophasphamide；immunoregulotory activity；mice?　　杜仲总多糖(eucommia ulmoides oliv polysaccharides,eop)是从杜仲的干燥树皮中提取出的水溶性成分,是近年来发现的杜仲又一活性成分。本实验观察杜仲总多糖对传统化疗药物环磷酰胺致免疫抑制小鼠的影响,并初步探讨其作用机制,为杜仲的进一步开发利用提供实验依据。　　1? 实验材料　　1.1? 动物 ??? 昆明种清洁级8周龄小白鼠60只,体重(21±4)g,雌雄各半,泰山医学院实验动物中心提供,合格证号：鲁动质字20103；标准饲料喂养,实验前饲养5 d。　　1.2? 药物与试剂 ??? 杜仲中药饮片,由本院中药学教研室高红莉老师鉴定为正品；环磷酰胺粉针剂,上海华联制药有限公司生产,批号041109(用时以生理盐水配成2 mg/ml溶液)；香菇多糖冻干粉,福建梅峰制药厂生产,批号041223(用时以生理盐水配成0.5 mg/ml溶液)。鸡血红细胞混悬液,用时取新鲜鸡血5 ml,离心去上清液,用生理盐水洗涤2次,再以生理盐水配成1%混悬液。　　1.3? 仪器 ??? 中草药粉碎机,fw/t型,天津市泰斯特仪器有限公司；re-52a型旋转蒸发器,上海亚荣生化仪器厂；shb-1395a型循环水式多用真空泵,郑州长城科工贸有限公司；spectrumlab54紫外可见分光光度计