伊曲康唑脂质体体外抗真菌作用研究.doc

伊曲康唑脂质体体外抗真菌作用研究 魏华董迪宋笑丹吴琳华* (1. 哈尔滨医科大学附属第二医院药学部；黑龙江省高校重点实验室，哈尔滨 150086哈尔滨医科大学附属第医院药学部，哈尔滨 1500 摘 要 目的研究伊曲康唑脂质体体外抗深部真菌作用。方法参照美国临床实验室标准化协会CLSI，曾经名为NCCLS推荐的M27-A2方案微量液基稀释法，测定伊曲康唑脂质体、伊曲康唑注射液的最小抑菌浓度MIC)和最小杀菌浓度MFC)，采用平皿菌落计数法绘制杀菌曲线。结果伊曲康唑脂质体与伊曲康唑注射液相比，对受试菌株的MIC、MFC和杀菌曲线相似。结论伊曲康唑脂质体保持了与市售伊曲康唑注射液相似的体外抑菌、杀菌活性，具有良好的体外抗真菌作用。 关键词 伊曲康唑；脂质体；最小抑菌浓度MIC)；最小杀菌浓度MFC)；杀菌曲线 In Vitro Antifungal Activity of Itraconazole Liposome WEI Hua，DONG Di，SONG Xiao-dan，WU Lin-hua* (1. Department of Pharmacy, the Second Affiliated Hospital of Harbin Medical University, Key Laboratory of College in Heilongjiang Province, Harbin 150086, China; 2. Department of Pharmacy, the First Affiliated Hospital of Harbin Medical University, Harbin 150001, China) ABSTRACT：OBJECTIVE To study the antifungal activity of Itraconazole Liposome in vitro. METHODS According to the reference M27-A2 broth microdilution method recommended by the clinical and laboratory standards institute (CLSI; formerly NCCLS) subcommittee, assay the minimal inhibitory concentration (MIC) and the minimal fungicidal concentration (MFC) of Itraconazole Liposome and Itraconazole Injection, killing curve was drown by calculating the colony number . RESULTS MIC, MFC and killing curve of Itraconazole Liposome against 56 fungi were similar to those of Itraconazole Injection in experiments. CONCLUSION Itraconazole Liposome retained similar antifungal activity as Itraconazole Injection. Itraconazole Liposome has significant action in inhibiting fungi and fungicidal effect and excellent antifungal activity in vitro study experiments. KEY WORDS： itraconazole；liposomesthe minimal inhibitory concentration (MIC) ;the minimal fungicidal concentration (MFC) ;killing curve 伊曲康唑（itraconazole，ITZ）是三唑类广谱抗真菌药物，通过抑制真菌细胞膜必成分麦角固醇的生物合成而发挥其抗真菌作用[]，对深部真菌感染疗效较好是近数十年来抗真菌很大进展[]。伊曲康唑注射液长期大量应用会造成体内药物蓄积，将伊曲康唑制成脂质体的剂型可以更好地发挥其疏水性，减少用药剂量，提高其生物利用度，提高伊曲康唑在靶器官的浓度[]。本实验考察了伊曲康唑脂质体对临床常见五种深部真菌体外抗真菌，并与市售伊曲康唑注射液进行比较，为进一步开发伊曲康唑脂质体提供依据。 1 材料 1.1 药物与试剂 伊曲康唑原料药寿光富康制药有限公司，纯度99