拟南芥中几个热相关miRNAs的响应热处理的表达分析.docVIP

拟南芥中几个热相关miRNAs的响应热处理的表达分析 拟南芥中几个热相关miRNAs的响应热处理的表达分析(6300字) 摘 要：人类的生存和发展离不开自然界中的植物，了解植物适应温度变化的分子机理对作物种质创新具有重要的理论和实际意义。miRNA作为生物体内基因表达的重要调控元件,在植物适应外界环境和生长发育 ... p拟南芥中几个热相关miRNAs的响应热处理的表达分析(6300字)br / 摘nbsp; 要：人类的生存和发展离不开自然界中的植物，了解植物适应温度变化的分子机理对作物种质创新具有重要的理论和实际意义。miRNA作为生物体内基因表达的重要调控元件,在植物适应外界环境和生长发育的过程中起着重要的调控作用,然而对植物体内与热胁迫相关的miRNA还不是十分清楚。本研究通过生物信息学和RT-PCR的方法分析热相关的miRNA的转录表达,为进一步了解miRNA在温度胁迫的生物学功能奠定基础。br / 关键词：拟南芥；胁迫；miRNAbr / br / Several Heat-related miRNAs In Arabidopsis Response to The Expression of Heat Treatmentbr / Abstract：Inseparable from human existence and development of plants in nature, to understand plant adaptation to the molecular mechanism of temperature change on crop germplasm has important theoretical and practical significance. miRNA gene expression in vivo as an important regulatory elements in plant adaptation to the external environment and the process of growth and development plays an important regulatory role, however, heat stress on plants associated with the miRNA is not very clear. In this study, bioinformatics and RT-PCR method to analyze the transcription of heat-related miRNA expression of miRNA in order to further understand the biological function of temperature stress basis. br / Keywords：Arabidopsis thaliana；Stress；miRNA /p pP/P p扶桑绵粉蚧田间种群消长动态研究(7500字)br / 摘nbsp; 要：在自然条件下通过在湖南农业大学实习基地不同地点的调查研究发现了扶桑棉粉蚧在农大的发生规律。了解了影响扶桑绵粉蚧种群消长的影响因素有：不同寄主、气象因素、捕食性天敌、寄生性天敌及其他昆虫，鉴定了扶桑绵粉蚧雄成虫、雌成虫、一龄，二龄、三龄若虫的形态特征。并组建自然温度条件下该虫种群消长动态图。实验结果表明：暴雨、捕食性天敌、寄生性天敌对扶桑绵粉蚧的种群数量增长有抑制作用，蚂蚁对扶桑绵粉蚧种群数量增长有促进作用。扶桑绵粉蚧在芙蓉花和苍耳上的扶桑绵粉蚧生长情况种群消长动态及寄主死亡时间也不尽相同。br / 关键词：种群消长 不同寄主 天敌 气象因素br / br / The study on the dynamic growth and decline of the population of the field Phenacoccus solenopsis Tinsleybr / Abstract: in the natural conditions in Hunan Agricultural University base through the investigation and study different sites found Phenacoccus solenopsis Tinsley in the occurrence of pest regularly. Currently Master influence the pest population growth Phenacoccus solenopsis Tin