抗稻瘟病基因pi9的sts连锁标记开发及在分子标记辅助育种中的应用.pdfVIP

(Chin J Rice Sci), 2011, 25( 1) : 25~ 30 http: / / w w w . r icesci. cn DO I: 10 . 3969/ j. issn . 1001-72 16. 2011. 01. 004 25 P i9 STS 殷得所 夏明元 李进波 万丙良 査中萍 杜雪树 戚华雄* ( , 430064; * , E-mail: qihx@ hb aas. com) Developm ent o ST S M ar ker L inked to Rice Blast Resistance Gene P i9 in M ar ker- A ssisted Selection Br eeding * YI N De- suo , XIA M ing-y uan, L I Jin- bo, W AN Bing- liang, ZH A Zhong- ping , DU Xue- shu, Q I Hua-xiong (Food Crop s I nstitute, H ubei A cademy of A gricultural Sciences , Wuhan 4 0064 , China; * Corresp onding author, E-mail : qihx@ hbaas . com) Y IN Desuo, XIA M ingyu an , LI Jinbo, et al. Development o ST S marker linked to rice blast r esistan ce gene Pi9 in m ar ker- assisted selection breeding. Chin J Rice Sci, 2011, 25( 1) : 25- 30. Abstract: In dica rice lin e 75- 1- 127 carrying broad- sp ectrum rice blast r esist ance gene Pi9 w as used as resist ance gene donor or gen etic im provem ent o Yangdao 6 and R6547 . By alignm ent o genomic sequences r om th e Pi9 locus o 75- 1- 127, Yangdao 6 an d Nipp onb ar e, co- dominant ST S ( sequence- t agged site) mar ker PB9- 1, w h ich linked to th e Pi9 gene, w as developed or m o- lecular marker- assisted selection ( MA S) . E ight b ackcross lin es carry ing Pi9 w er e obt ain ed, am ong w hich our lines cont ained th e genetic backgr ou nd o Yangdao 6 and the oth er our lines h ad th e R6547 b ackg roun d. U nder natur al disease in ection condi- tion s in Enshi Cou nty an d Yichang Cou nty o Hub ei Provin ce, Chin a, the Pi9- car rying lines sh ow ed hig her blast disease resist- an ce th an Yangdao 6 and R6547 in variou s degrees. T h e hy br