蹄蝠科3属蝙蝠mtDNA控制区的分子特征与进化.docVIP

  蹄蝠科 3 属蝙蝠 mtDNA 控制区的分子特征 与进化# 孙克萍1,2，罗丽2，刘森2，张桢珍2，冯江1,2** 5 10 15 20 25 30 35 （1. 吉林省动物资源保护与利用重点实验室，长春 130024； 2. 东北师范大学城市与环境科学学院，长春 130024） 摘要：翼手目蹄蝠科蝙蝠线粒体控制区结构与进化特征少有研究，为了给出蹄蝠控制区详尽 的进化特征，本研究首次测序并分析了蹄蝠科 3 属蝙蝠的控制区全序列。蹄蝠科蝙蝠控制区 具有典型的哺乳动物特征，可划分为 ETAS、CD 和 CSB 结构域。在 ETAS 区，检测到 ETAS1 和 ETAS2 保守元件，前者变异度（41.9%）低于后者（51.7%）；在三叶蹄蝠和双色蹄蝠内 检测到长重复元件（分别为 26 bp 和 80 bp），可形成稳定的二级结构。在 CD 区，共检测 到 5 个保守元件，F、E、D、C 和 B。在 CSB 区内，共检测到 CSB1、CSB2、CSB3 和 CSB1-like 元件。CSB2 变异度最高（44.4%），CSB3 最保守。在 CSB 内还检测到长短不等的串联重 复元件（6 bp、8 bp、16 bp 或 20 bp），蹄蝠科 3 个属间重复序列的长度、碱基组成和拷贝 数均存在差异。 关键词：分子生物学；蹄蝠；控制区；保守元件；重复序列 中图分类号：Q78 Molecular characteristics and evolution of the mitochondrial control region in three genus of Hipposideridae SUN Keping1,2, LUO Li2, LIU Sen2, ZHANG Zhenzhen2, FENG Jiang1,2 (1. Jilin Key Laboratory of Animal Resource Conservation and Utilization, ChangChun 130024; 2. School of Urban and Environmental Sciences,Northeast Normal University, ChangChun 130024) Abstract: There have been few studies of the structural and evolutionary characteristics of the mitochondrial control region (CR) in hipposiderids. The CR was firstly sequenced for three genera in Hipposideridae to give a detailed overview of its features. The CR of leaf-nosed bats was divided into three domains (ETAS, CD and CSB) like that of other mammals. In the ETAS domain, ETAS1 and ETAS2 elements were detected, and the variability of ETAS1 (41.9%) was lower than that of ETAS2 (51.7%). Repeated motifs were examined in Aselliscus stoliczkanus (26 bp) and Hipposideros bicolor (80 bp) in ETAS, which can be formed the stable secondary structures. In the CD domain, 5 conserved blocks, F, E, D, C and B, were found. In the CSB domain, CSB1, CSB2, CSB3 and CSB1-like elements were detected in all species. The CSB2 element had the highest variability (44.4%), but the CSB3 was the most conserved, with no variable site. Repeated motifs were present in the CSB in all individuals (6 bp, 8 bp, 16 bp or 20 bp), but the length, base compos