乳清酸对大鼠与小鼠肝脏脂质代谢影响的比较研究.docVIP

  乳清酸对大鼠与小鼠肝脏脂质代谢影响的 比较研究# 刘春花，任兵兴，王静凤，王玉明，徐杰，薛长湖** 5 （中国海洋大学食品科学与工程学院，青岛 266003） 摘要：目的 研究乳清酸对大鼠和小鼠肝脏脂质代谢影响的差异，并探讨其机制。 方法 将 Wistar 大鼠和 BALB/c 小鼠按体重分为对照组和乳清酸组，对照组喂食标准饲料，乳清酸组 喂食 1%乳清酸饲料 10 d 后，分别测定肝脏 TG、TC 水平及肝脏脂肪合成分解相关酶的活性 10 （FAS、CPT 及过氧化物酶体β氧化），同时测定血清 TG、TC、HDL-C 水平。 结果 大 鼠喂食乳清酸 10 d 后，肝脏 TG、TC 水平分别升高 4.8 倍（P0.01）和 1 倍（P0.01），肝 脏 FAS 活性显著提高，CPT 活性显著降低，同时血清 TG、HDL-C 水平也显著提高。而小 鼠喂食乳清酸后肝脏 TG、TC 及血清 TG、TC、HDL-C 水平未见显著变化，但肝脏 FAS 活 性、过氧化物酶体β氧化活性显著下降，同时 CPT 活性有升高趋势。 结论 乳清酸对大鼠 15 20 25 30 35 40 小鼠肝脏脂质代谢的不同影响与肝脏脂肪合成分解相关酶的活性变化有密切关系。 关键词：食品营养学；脂肪肝；乳清酸；脂肪酸合成酶；肉碱棕榈酰转移酶 中图分类号：Q95-33 Comparison of the effects of orotic acid on liver fatty acid accumulation in rats and mice Liu Chunhua, Ren Bingxing, Wang Jingfeng, Wang Yuming, Xu Jie, Xue Changhu (College of Food Science and Engineering, Ocean University of China, Qingdao 266003) Abstract: Objective To study the different effects of orotic acid (OA) on liver lipid metabolism in rats and mice, and investigate their different mechanisms. Methods Wistar rats and Balb/c mice were randomly divided into control groups and OA groups (8 animals in each group), respectively. The control groups were fed with standard diet, while the OA diet (standard diet plus 1% OA) for 10 days. Hepatic triglyceride (TG), total cholesterol (TC) and activities of key enzymes related to lipids metabolism, as well as serum TG, TC and HDL-C levels were determined. Results Hepatic TG, TC levels in the OA-fed rats were increased 4.8-fold (P0.01) and 1-foldand (P0.01) than those of the rats control group, liver fatty acid synthase (FAS) activity was elevated significantly, carnitine palmitoyl transferase (CPT) activity was decreased, and serum TG, TC and HDL-C levels were increased as well. However, there were no changes in hepatic TG, TC, HDL-C and serum TG, TC in the OA-fed mice, and the liver FAS activity and peroxisomal β-oxidation were decreased markedly. Conclusions There are distinct differences between the lipid metabolisms in orotic aci