基于均匀设计与支持向量回归的棉铃虫幼虫全纯人工饲料配方优化.docVIP

  基于均匀设计与支持向量回归的棉铃虫幼 虫全纯人工饲料配方优化# 周世豪1,2，李俊2，姚润贤1,2，张星1,2，袁哲明1,2** 5 10 15 20 25 30 35 40 （1. 湖南省作物种质创新与资源利用重点实验室,长沙 410128； 2. 湖南农业大学生物安全科学技术学院,长沙 410128） 摘要：昆虫全纯人工饲料配方是精细研究昆虫营养生理的前提。本研究以棉铃虫 Helicoverpa armigera 幼虫为供试对象，以本室前期发展的多因素多水平配方优化实验设计与分析方法 UD-SVR 为指导，以略加修改的苹浅褐卷蛾 Epiphyas postvittana 幼虫全纯人工饲料为初始配 方，先优化 20 种天然氨基酸，再优化蔗糖等其他 8 种非氨基酸营养组分，经 4 轮 97 个处理 组合，获得一个棉铃虫幼虫全纯人工饲料满意配方，平均蛹重、化蛹率分别由初始配方的 0.160 g 和 10.4 ％高效增至满意配方的 0.255 g 和 97.9 ％。该全纯人工饲料满意配方的获得， 不但再次证实了 UD-SVR 方法的有效性，同时为进一步深入研究棉铃虫营养需求与营养代 谢奠定了基础。 关键词：棉铃虫；全纯人工饲料；均匀设计；支持向量回归；配方优化 中图分类号：Q965 Optimization chemically defined diet for the cotton bollworm based on uniform design and support vector regression ZHOU Shihao1,2, LI Jun2, YAO Runxian1,2, ZHANG Xing1,2, YUAN Zheming1,2 (1. Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization, Hunan Agricultural University, Changsha 410128, China; 2. College of Bio-safety Science and Technology, Hunan Agricultural University, Changsha 410128, China) Abstract: In order to get a satisfying formula of the chemically artificial diet for the cotton bollworm, Helicoverpa armigera larvae, the current paper used UD-SVR, an experimental design and analysis method reported by our preliminary studies, for formula optimization. Based on the benchmark diet of the chemically artificial diet for the larvae of the light brown apple moth, Epiphyas postvittana, the contents of 20 natural amino acids were optimized first, and the contents of 8 non-amino acid nutrients including sucrose were optimized then. After 4 rounds of optimization with total 97 schemes, an optimal formula of the chemically artificial diet for the cotton bollworm larvae was obtained. Under this diet, the mean pupal weight and the pupation rate were 0.255 g and 97.9 % which exceeds prominently from 0.160 g and 10.4 % under the benchmark diet. The result reconfirmed the effectiveness of UD-SVR in the complex prescription optimization. More importantly, the optimal chemically artificial diet for th