直接固相微萃取和顶空固相微萃取联合分析烟草香气组分.docVIP

  直接固相微萃取和顶空固相微萃取联合分 析烟草香气组分# 舒建军，张珊，刘名茗，李婵** 5 10 15 20 25 30 35 40 （华中农业大学资源与环境学院，武汉 430070） 摘 要 ： 应 用 自 制 的 1- 三 乙 氧 基 硅 丙 基 -3- 甲 基 咪 唑 六 氟 磷 酸 - 羟 基 硅 油 （[TESPmin]PF6-OH-TSO）溶胶-凝胶探头，采用先超声萃取-直接固相微萃取，后顶空固相 微萃取，气相色谱联用的方法对 2 种不同产地烟草样品以及 2 种相同产地、不同陈化时间的 烟草样品中的香气组分进行了分析。对不同分析方法的萃取条件进行了优化。超声萃取-直 接固相微萃取的最佳萃取条件是超声萃取 30min，然后在 60条件下直接萃取 30min；顶空 固相微萃取的最佳萃取条件是 90萃取 50min。顶空固相微萃取对保留时间在 25～40min 范围内的物质有较高的萃取效率，而超声萃取-直接固相微萃取则对保留时间在 38～50min 的物质有较高的萃取效率。不同产地的样品，其芳香组分在种类和含量上均存在较大差异， 而相同产地的样品，仅在含量上存在较大差异。该方法具有操作简单、快速、重现性好、费 用少等特点, 为烟草香气组分的分析提供了一种可供参考的方法。 关键词：固相微萃取；气相色谱；烟草香气组分 中图分类号：O658.2；O657.7+1 The combined direct- and headspace-solid phase microextraction for the analysis of tobacco flavor constituents Shu Jianjun, Zhang Shan, Liu Mingming, Li Chan (College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070) Abstract: In this paper, the sol-gel derived 1-(3-triethoxysiyl propyl)-3-methyl imidazolium hexafluorophosphate-hydroxy-terminated silicone oil（TESPMIM[PF6]-OH-TSO）fiber was used to analyze flavor constituents in tobaccos obtained from two different producers and two identical producers with different aging times by ultrasonic extraction-direct solid-phase microextraction (UE-DI-SPME) combined with headspace solid-phase microextraction (HS-SPME) and gas chromatography (GC). The optimum sampling conditions of these two extraction modes were investigated. The results showed that the optimal conditions for HS-SPME is 50min at 90℃, and for UE-DI-SPME is 30 min at 60℃ after ultrasonic extraction 30min. Among them, the HS-SPME had higher sensitivity for the compounds whose retention times were at 26～40min, and the UE-DI-SPME had higher sensitivity for the compounds whose retention times were at 38～50min. For the tobaccos obtained from different areas, the species and concentration of the flavor constituents were different, and for the tobaccos obtained from the same areas, the species of the flavor constituents were similar but their concentrat