黄芩苷、槲皮素对LPS致流产小鼠蜕膜细胞损伤的保护作用.docVIP

  黄芩苷、槲皮素对 LPS 致流产小鼠蜕膜细胞 损伤的保护作用# 李云霞1，赵延涛1，钟秀会2** 5 10 （1. 河北农业大学动物医学院，河北 保定 071001； 2. 河北农业大学中兽医学院，河北 保定 071001） 摘要：为了探讨黄芩苷、槲皮素对 LPS 致流产小鼠蜕膜细胞损伤的保护作用及保胎效果，以 及对血清 TNF-α 和孕酮含量的影响,应用蜕膜细胞培养技术观察黄芩苷、槲皮素对小鼠蜕 膜细胞损伤的保护作用，用 HE 染色观察对蜕膜组织形态的影响，用 ELISA 检测血清 TNF-α、 孕酮含量的变化。结果显示，黄芩苷、槲皮素对蜕膜细胞的损伤均有良好的保护作用，对 LPS 致流产均有良好的保胎效果，槲皮素效果更优。黄芩苷、槲皮素有效较低血清 TNF-α的 含量（P 0.01），对孕酮含量无影响。 关键词：临床兽医学；黄芩苷；槲皮素；LPS；蜕膜细胞；保胎 中图分类号：S853.9 15 Protective effects of baicalin and quercetin on decidua cells in LPS induced abortion mice Li Yunxia1, Zhao Yantao1, Zhong Xiuhui2 (1. College of Veterinary Medicine,Agricultural University of Hebei, HeBei BaoDing 071001; 20 25 30 35 40 2. College of Traditional Chinese Veterinary Medicine, Agricultural University of Hebei, HeBei BaoDing 071001) Abstract: The study was carried out to compare about the protective effects of baicalin and quercetin on decidual cells of abortion mice induced by LPS，using a mouse model in vitro and in vivo. Decidual cells were cultured in vitro, and randomly divided into blank group, LPS group, LPS+baicalin group, and LPS+quercetin group. Pregnant female mice were divided into 8 groups for immunohistochemistry staining, and detecting the TNF-α and progesterone by enzyme linked immunosorbent assay (ELISA).The results clearly revealed that baicalin, quercetin can effectively prevent abortion and injuries to decidual cells induced by LPS. The effect of quercetin was more significant. TNF-α was decreased lower than the LPS group(p 0.01). There was no significant influence on the progesterone levels. Keywords: Clinical veterinary medicine; baicalin; quercetin; LPS; decidua cells; anti-abortive 0 引言 自发性流产是早期妊娠失败导致胚胎死亡的常见形式。疾病、感染和各种物理和化学因 素，还有各种细胞因子均可导致妊娠的失败。目前，许多研究着眼于子宫蜕膜细胞，蜕膜细 胞在妊娠的发展和维持上起着至关重要的作用[1]。蜕膜是胚泡植入后的子宫内膜在雌激素与 孕激素及溶解产物等作用下的母面组织，与子面滋养层共同构成母胎界面[2]。为了适应孕体 的生长发育，一定数量的蜕膜细胞必须死亡[3]。但过度损伤的蜕膜细胞终将不利于胚胎的营 养供给和免疫微环境的维持，从而导致妊娠失败。 细菌脂多糖通过 Toll 样受体导致早期妊娠失败，已经用于建立小鼠流产的模型[4]。细菌 脂多糖（革兰氏阴性细菌细胞壁组成成分）是潜在的炎症刺激原，它导致的早产、自发性流 基金项目：高等学校博士学科点专项科研基金资助（