鸭儿芹提取物抗氧化和保肝作用研究.docVIP

  鸭儿芹提取物抗氧化和保肝作用研究# 李娟1,3，谢运昌1，蒋小华1，宁德生1，刘安韬2** （1. 广西植物研究所，广西 桂林 541006； 5 10 15 20 25 30 35 40 2. 广西卫生干部管理学院，南宁 530021； 3. 暨南大学，中药及天然药物研究所，中药药效物质基础及创新药物研究广东省高校重点 实验室，广州 510632） 摘要：目的：研究鸭儿芹提取物的体外抗氧化和对小鼠急性肝损伤的保护作用。方法：通过 测定鸭儿芹提取物清除羟自由基、超氧阴离子自由基活力及总抗氧化活力，评价其体外抗氧 化作用，采用 CCl4 和酒精诱导小鼠急性肝损伤，测定小鼠血清 AST 和 ALT，同时测定肝组 织中 SOD、MDA 氧化指标以评价体内保肝作用。结果：鸭儿芹总提取物和正丁醇提取物高剂 量有较好的抗氧化作用，并且能降低急性肝损伤小鼠血清 AST 和 ALT 含量，且降低肝组织中 的 MDA 含量，升高肝组织中 SOD 含量。结论：鸭儿芹提取物具有抗氧化和保肝作用，且其抗 氧化和清除自由基作用是其保肝作用机制之一。 关键词：鸭儿芹；抗氧化；小鼠；急性肝损伤 中图分类号：R961 Hepatoprotective Effect and Antioxidant Activity of Cryptotaenia japonica Hassk. LI Juan1,3, XIE Yunchang1, JIANG Xiaohua1, NI Desheng1, LIU Antao2 (1. Guangxi Insitute of Botany, Guangxi Zhuangzu Autonomous Region and the Chinese Academy of Science, GuangXi GuiLin 541006; 2. Guangxi Health Techological College, NanNing 530021; 3. Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine and New Drugs Research, Institute of Traditional Chinese Medicine and Natural Products, Jinan University, GuangZhou 510632) Abstract: OBJECTIVE To study hepatoprotective effect and antioxidant activity of Cryptotaenia japonica Hassk. METHODS The antioxidant activity of the extracts of Cryptotaenia japonica was evaluated by detecting hydroxy radical, superoxide radical anion and total anti oxidant activity in vitro. The mouse model of acute hepatic injury was established by giving CCl4 abdominally and infusing alcohol into stomach. The activity of ALT and AST in serum and the levels of MDA and SOD in liver tissue were examined to evaluate the hepatoprotective and antioxidant activity of the extracts. RESULTS The total extract and n-butanol extract of Cryptotaenia japonica has good activity of antioxidant in vitro. And they could reduce the activities of ALT and AST in serum and the levels of MDA in liver tissue, and raise the levels of SOD in liver tissue. CONCLUSION The results showed that the extract of Cryptotaenia japonica had a good hepatopr