降酚真菌的分离培养.pdf

降 酚 真 菌 的 分 离 培 养 降 酚 真 菌 的 分 离 培 养 摘 要 含酚污水在我国水污染治理中被列为重点解决的有害污水之一，它具有浓度高、排放 量大、涉及面广等突出特点。它的大量排放给环境造成了严重污染。本实验利用真菌处理 废水中酚类物质，使其浓度降低。为了获得高效降酚优势真菌，以北京燕山石化集团公司 炼油厂轻油罐区的活性污泥为菌源，驯化、筛选、分离出4 中以苯酚为唯一碳源和能源的 降酚真菌。驯化采用好氧条件下一次性加入大剂量苯酚和定时逐渐加量的方法，优势菌种 初步鉴定为假丝酵母属。然后以温度、pH 值、NaCl 浓度为主要因素做正交实验，得出适 合菌种生长的条件。温度对菌体生长影响最大，pH 值和盐浓度的影响较小且接近，在 30 ℃最适合菌体生长。 优势真菌分离培养的成功，为驯化、诱变真菌作了良好的开端，为彻底治理废水对环 境的污染打下了坚实的基础。 关键词：酚 真菌 生物降解 分离 正交 1 降 酚 真 菌 的 分 离 培 养 Separation and Incubation of Phenol-degrading fungi Yu Xiaolu (Department of Chemical Engineering, Beijing Institute of Petro-chemical Technology. Post-code 102600) ABSTRACTS Because of the properties of high concentration, large discharge quantity, wide range to touch on, the phenol contained wastewater was listed as an emphasis harmful polluted water to be settled in the polluted water treatment. The large quantity of its discharging had made seriously pollution. In laboratory, we utilized fungi to treat phenol contained wastewater droping the concentration. Four kinds of strains with phenol as the only origin of carbon and energy have been obtained from the activated sludge of wastewater treatment plant of the area of light oil tunk-farm in Beijing Yanshan petrochemical GroupCorporation via acclimatization, screening and separation. The acclimatization was done in aerobic condition with great dosage of phenol added by one time and grdually increased dosage of phenol in constant time. The strain