水杉基因组微卫星分析及标记开发.pdfVIP

第 49卷 第 6期 林 业 科 学 Vo1．49．No．6 2013年 6月 SCIENTIA SILVAE SINICAE Jun．，20 13 doi：10．11707／j．1001-7488 水杉基因组微卫星分析及标记开发 张新叶 张亚东 彭 婵 宋丛文 杨彦伶 (1．湖北省林业科学研究院 武汉 430075；2湖北生态工程职业技术学 院 武汉 430200) 关键词 ： 水杉 ；基 因组 ；微卫星 ；标记开发 中图分类号：$718．46 文献标识码 ：A 文章编号：1001—7488(2013)06—0160—07 AnalysisofGenomicM icrosatelliteSequenceandDevelopmentofSSR M arkersinM etasequoiaglyptostroboides ZhangXinye ZhangYadong PengChart SongCongwen YangYanling (1．HubeiForestryAcademy Wuhan430075； 2．HubeiEcologyVocationalCollege Wuhan430200) Abstract： Inthispaper，thepartialgenomeofMetasequoiaglyptostroboides，arareplant，wassequencedbyusingthe ROCHE-454GLX high—throughputsequencingplatform．Through sequenceassembly andmicrosatellite finding，1965 microsatellitelociwereobtained inthesequenceandtherepeatunitlengthwas2 5basepairs，bywhich921pairsof primerwere designed with the Primer 3 Plus software． Analysis ofthese microsatellite sequences showed that tetranucleotidemicrosatellitewasthemostabundant，accountingfor38．8％ ofthe totalrepeatsequences，followedby dinucleotide(31．8％)，trinucleotide(22％)andpentanucle0tide(7．4％)intheM．glyptostroboidesgenome．Among the dinucleotiderepeattypes，AG typewasthemost，accountingfor13．9％ oftotalrepeatsand43．8％ ofdinucleotide repeats．Intheeighttrinueleotiderepeattypes．AAG typeaccountedfor8．3％ oftotalrepeatsand37．7％ oftrinucleotide repeats，followedbyATG (23．1％)，AAC (16．7％)andAAT (13．0％)．Theanalysisofdifferentlengthsofthe microsatelliterepeatunit showed thatthe mostabundantvariantswere dinucleotidemicrosatellite and there were23 differenttypesofrepeatlengths，followedbythetetranucleotiderepeat(10types)，trinucleotiderepeat(8types)and pentanucleotiderepeat(3types)．Thevalidationof