血吸虫基因操作研究进展.pdfVIP

第21卷 第4期 生命科学 Vol. 21, No. 4 2009年8月 Chinese Bulletin of Life Sciences Aug., 2009 文章编号 ：1004-0374(2009)04-0579-05 血吸虫基因操作研究进展 陈　晶， 林矫矫，蔡幼民，程国锋* (中国农业科学院上海兽医研究所，农业部动物寄生虫学重点开放实验室，上海 200241) 摘　要：近年来，血吸虫基因组、转录组、蛋白质组和分泌组研究广泛开展，迫切需要针对单个分 子功能深入研究。开展血吸虫基因操作研究不仅可在虫体内深入研究基因功能，对进一步理解血吸虫生 长发育机理和寄生生活特征具有重要意义，还可建立抗血吸虫候选疫苗和药物靶标筛选的重要平台。为 此，本文总结基因操作技术在血吸虫学中的应用并分析其现状。 关键词：血吸虫；基因操作；疫苗；药物靶标 中图分类号：S85; R18　　文献标识码：A Advances in genetic manipulations of schistosomes CHEN Jing, LIN Jiao-jiao, CAI You-min, CHENG Guo-feng* (Key Laboratory of Animal Parasitology, Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese 　 Academy of Agricultural Sciences, Shanghai 200241, China) Abstract: There is a great need for deeply understanding the functions of single molecule of schistosomes in vivo due to the accumulated information from schistosome genomics, transcriptomics and proteomics. The studies on genetic manipulations of schistosomes not only provide the techniques for further investigating gene functions of schistosomes in vivo, which could help not only to understand the mechanism of schistosome growth and development as well as parasitic characters, but also establish the platform for screening novel vaccine candidates and/or drug targets for controlling schistosomiasis. Therefore, we reviewed the advances in genetic manipulations of schistosomes, its application, and its current status. Key words: schistosome; genetic manipulation; vaccines; drug targets 日本血吸虫病人畜共患，危害严重，是我国 复杂，无论是早期的虫体抗原苗、辐射致弱童虫 最重要的公共卫生问题之一，与艾滋病、肝炎、