超临界二氧化碳梯度结晶穿心莲内酯的纯化工艺研究.pdfVIP

中国农业科学 2004,37(12):2047-2050 Scientia Agricultura Sinica 超临界二氧化碳梯度结晶穿心莲内酯的纯化工艺研究 1 1 1 2 张文成 潘 见 谢慧明 陈克勋 1 合肥工业大学农产品生物化工教育部重点实验室 合肥 230069 2 中国科学技术大学高分子科学与工程系 合肥 230026 摘要 采用单因素试验法探讨超临界CO2 梯度结晶压力 温度 时间对穿心莲内酯纯度的影响 并利用高效 液相色谱进行纯度检测 结果表明 超临界CO2 在萃取穿心莲内酯时 出现同步结晶 且在结晶板上呈梯度分布 选择较佳纯化工艺参数为压力20 MPa 温度55 时间90 min CO2 流量15L·min-1 时 得到穿心莲内酯的纯度 达到80% 以上 关键词 超临界二氧化碳 梯度结晶 穿心莲内酯 高效液相色谱 Study on Purifying Technology of Andrographolide by Supercritical Carbon Dioxide Gradient Crystallization 1 1 1 2 ZHANG Wen-cheng , PAN Jian XIE Hui-ming , CHEN Ke-xun 1 2 ( Key Laboratory of Bioprocess of State Education Ministry of Hefei University of Technology, Hefei 230069; School of Chemistry and Material, University of Science and Technology of China, Hefei 230026) Abstract Effects of gradient crystallizing pressure, temperature and time on the purity of andrographolide were investigated via single factor experiments, and the purity of andrographolide was by HPLC. The result showed that andrographolide could be synchronously extracted and crystallized in extraction container by supercritical CO2 extraction. Moreover, the purity of andrographolide crystal was distributed gradiently on crystallization board. When the optimized parameters of pressure 20 MPa, temperature 55 , and time 90 min were selected, the content of obtainedandrographolide was more than 80%. Key words: Supercritical carbon dioxide; Gradient crystallization; Andrographolide; High performance liquid chromatography (HPLC) 穿心莲[Androg