神经网络优化富集EPA和DHA甘油酯的工艺研究.pdfVIP

44 CHINA OILS AND FATS 2013 Vol. 38 No. 7 油脂化学 神经网络优化富集EPA 和DHA 甘油酯的工艺研究 黄万有，李德涛 ，刘书成，郝记 明，张 静 ( 广 东海洋大学 食品科技 学院，广东省水产品加工与安全重点 实验室，广东普通 高校 水产品深加工重点 实验室，广东 湛江 524088) 摘要:以军曹鱼内脏油为原料，采用脂肪酶 OF 水解法富集鱼油 中 EPA 和 DHA 甘油酯，应用神经 网 络优化其水解工艺参数 。结果表明: 在缓冲溶 液 pH 7. 0 反 应体 系中，水 油质量 比 3 ∶ 1、反 应 温度 40 ～ 45 ℃ 和脂肪酶用量 1. 5% ，富集鱼油 中 DHA 与 EPA 总含量可达 32. 10% ，富集的鱼油色泽亮 黄 ，澄清透明，具有淡的鱼腥味 ，理化指标达到了 SC / T 3502—2000 精制鱼油的二级标准 。 关键词:神经 网络; 鱼油; 脂肪酶; EPA; DHA; 富集 中图分类号:TS225 ． 6 ;S937 ． 3 文献标志码:A 文章编号:1003 － 7969 (20 13)07 － 0044 － 05 Optimization of enrichment process of DHA and EPA glycerides using neural network HUANG Wanyou ，LI Detao ，LIU Shucheng ，HAO Jiming ，ZHANG Jing (Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution ， Key Laboratory of Aquatic Product Processing and Safety of Guangdong Province ，College of Food Science and Technology ，Guangdong Ocean University ，Zhanjiang 524088 ，Guangdong ，China) Abstract :EPA and DHA glycerides in fish oil from cobia viscera were enriched by lipase OF hydrolysis and the hydrolysis parameters were optimized using neural network． The results showed that when the mass ratio of water to oil was 3∶ 1，reaction temperature was 40 － 45 ℃ and dosage of lipase was 1. 5% in the reaction system of buffer solution (pH 7. 0)，the total content of DHA and EPA in the enriched fish oil was 32. 10% ． The enriched fish oil had the characteristics of brilliant yellow color ，clear and trans- parent with slight fishy smell ，and the physical and chemical indexes were accordance with the secondary standard of refined fish oil in SC / T 3502—2000 ． K