昆明山海棠抗HSV-1活性的研究.pdfVIP

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昆明山海棠抗HSV-1活性的研究.pdf

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HSV-1 HSV-1 HSV-1 MTTCPEPFU HSV-1 HSV-1 HSV-1 HSV-1 RT-PCR HSV-1 HSV-1 UL30UL39US6 (1) HSV-1 TC50 718.6µg/ml ED50 316.9µg/ml2HSV-1 IC50 62.5µg/ml106.3µg/ml 6.5µg/ml3 HSV-1 4 HSV-1 UL30UL39US6 UL30UL39 (1) HSV-1 HSV-1 2 βγ β HSV-1 I HSV-1 Abstact Object: To study the anti HSV-1 effect of different fractions of Tripterygium hypoglaucum Hutch, explore the anti HSV-1 mechanism of its crude alkaloids. Method: Using MTT, CPE, and PFU methods, firstly the aqueous extract of Tripterygium hypoglaucum Hutch was evaluated on the effect of anti HSV-1 activity; then different solvent-soluble fractions of the aqueous extract and its crude alkaloids were studied on the anti HSV-1 activity, so that the fractions with better activities could be filtrated;subsequently the fraction of n-butyl alcohol was selected to purify the monomer compounds, seeing about their anti HSV-1 activities; finally using PT-PCR, the mechanism of the crude alkaloids anti HSV-1 was studied, exploring the changes of the key genes of HSV-1: UL30UL39US6. Result: (1) The aqueous extract had the effect of anti HSV-1, and its TC50 and ED were 718.6µg/ml and 316.9µg/ml. (2) The fractions of n-butyl alcoholH O 50 2 and crude alkaloids had better activities, their IC50 were 62.5µg/ml106.3µg/ml and 6.5µg/ml. (3) The monomer compounds of n-butyl alcohol were belong to gl