可可茶、绿茶及其活性生物碱对拘束应激 小鼠脑内单胺神经递质的影响.docVIP

可可茶、绿茶及其活性生物碱对拘束应激 小鼠脑内单胺神经递质的影响 摘 要 目的：研究绿茶和可可茶及其活性生物碱成分咖啡碱和可可碱对拘束应激负荷下小鼠不同脑区内单胺类神经递质的影响。 方法：（1）采用拘束方法建立应激动物模型。（2）将实验动物随机分为14个组，即正常对照组(control)、应激模型组(model)、咖啡碱高、低剂量组(CH, CL)、可可碱高、低剂量组(TH, TL)，以及它们分别对应的拘束应激组(CHR, CLR, THR, TLR)，绿茶提取物、可可茶提取物空白组(S, P)及其相应拘束应激组(SR, PR)。咖啡碱、可可碱高、低剂量分别为30 mg/kg和10 mg/kg体重（3）采用高效液相色谱－电化学检测（HPLC-ECD）方法对实验动物脑皮质、下丘脑组织匀浆中NE、DOPAC、DA、HVA、5-HT、5-HIAA等单胺类递质及其代谢物进行测定。 结果：（1）咖啡碱能够明显提高正常及应激状态下小鼠脑皮质内NE、5-HT、5-HIAA及下丘脑内NE水平 (P0.05或P0.01)；可可碱能够提高正常及应激状态下小鼠脑皮质内5-HT 及5-HIAA(P0.01)水平，但其作用明显低于同剂量咖啡碱(P0.01)。（2）低剂量咖啡碱能够降低正常及应激状态下小鼠下丘脑DA水平 (P0.01)；高剂量咖啡碱能提高正常及应激时下丘脑DA水平（P0.05），两种效应均强于同等剂量可可碱(P0.05或P0.01)。（3）绿茶、可可茶水提物均提高正常及应激状态下小鼠脑皮质内NE、DA 5-HT、5-HIAA及下丘脑内NE、DA水平(P0.05或P0.01)。同时降低皮质和下丘脑中DOPAC、HVA水平(P0.01)；绿茶提取物对应激状态下下丘脑内NE、脑皮质内DA的升高作用，对下丘脑和皮质内5-HT、5-HIAA的升高作用明显强于可可茶提取物组(P0.05或P0.01)。 结论：（1）咖啡碱能够明显促进正常及拘束应激负荷下的小鼠脑内单胺类神经递质释放，可可碱作用与咖啡碱类似但强度较弱。（2）绿茶和可可茶水提取物均能够促进正常及应激负荷下小鼠脑内单胺类递质释放，可可茶的作用低于等剂量绿茶。 关键词：咖啡碱；可可碱；绿茶；可可茶；拘束应激；中枢单胺类递质 Effects of Camellia sinensis and Camellia ptilophylla and their purine alkaloids on central neurotransmitters in restrainted mice ABSTRACT Objective: To investigate the effects of Camellia sinensis and Camellia ptilophylla and their active purine alkaloids (caffeine and theobromine) on brain monoamine neurotransmitters in mice loaded with restraint stress. Methods: (1) The stress models were established by restraint method after a single administration of drugs or the vehicle. (2) Mice were randomly divided into 14 groups including nomal control group, model control group, caffeine and theobromine control groups and their stress groups at two doses (10 and 30 mg/kg), extract of Camellia sinensis and Camellia ptilophylla control groups（S，P） and their stress groups （SR，PR）at single dose of 5g/kg. (3) The concentrations of NE、DA、DOPAC、HVA、5-HT、5-HIAA in brain cortex and hypothalamus of mice were measured by high performance liqiud chromatography coupled with electrochemical detector (HPLC-ECD). Results: (1) Caffeine significantly increased