柑橘蜜挥发性成分的分析方法研究.docVIP

顶空固相微萃取-气/质联用技术 测定柑橘蜜挥发性成分 王方莉1 吴俐勤* 2 沈振陆1 莫卫民1 李丽1 钱鸣蓉2 章虎2 汪建妹2 1（浙江工业大学化学工程与材料学院，杭州 310014） 2 (浙江省农业科学院农产品质量标准研究所，杭州 310021） 摘要：建立柑橘蜜挥发性成分分析方法，采用顶空固相微萃取-气/质联用技术进行定性分析和半定量分析。前处理方法条件：纤维头DVB/CAR/PDMS，萃取温度50℃，平衡时间10min，萃取时间50min，解吸条件（255℃，3.5min），试样量5.00g，柑橘蜜和水的质量比为4：1；气-质联用技术分析检测。在8种柑橘蜜中鉴定96种化合物，主要有醇类、烃类、酮类、醛类、酯类，确定特征性成分为柠檬烯、反式氧化芳樟醇（呋喃型）、顺式氧化芳樟醇、β-芳樟醇和柠檬醛。各特征性成分相对标准偏差为2.9%-39.7%，此分析方法适合测定柑橘蜜挥发性成分。 关键词：柑橘蜜；挥发性成分；顶空固相微萃取；气/质联用技术 Determination of Volatiles of Citrus Honey by Headspace Solid Phase Microextraction-Gas Chromatography- Mass Spectrometry Wang Fang-li1,Wu Li-qin* 2,Shen Zhen-lu1,Mo Wei-min1,Li Li1,Qian Ming-rong2,Zhang Hu2,Wang Jian-mei2 1 (College of Chemical Engineering and Material Science, Zhejiang University of Technology, Hangzhou 310014, China) 2 (Institute of Quality and Standard on Agricultural Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China) Abstract: Methodology of volatile components of citrus honeys was developed. And markers, which used to discriminate adulterated citrus honeys, were ascertained. Volatiles were extracted by headspace solid phase microextraction(HS-SPME) ,separated and detected by gas chromatography-mass spectrometry(GC-MS).Sample preparation method was as follows: fiber DVB/CAR/PDMS, extraction temperature 50 ℃,equilibration time 10 min, extraction time 50 min desorption condition(255 ℃,3.5 min) , sample size 5.00 g and sample dilution ratio 4:1(w/w citrus honey to water). A total of 96 compounds were identified in eight citrus honeys, including alcohols, hydrocarbons, ketones, aldehydes and esters. Limonene, trans-linalool oxide (furanoid), cis-linalool oxide, β-linalool and citral were confirmed as potential markers. The relative standard deviations of each marker about absolute peak area were from 2.9% to 39.7%, which indicated that the method could be used to analysis volatiles, quickly label botanical origin and identify authentic unifloral citrus honey. Keywords: citrus honey; volatile compone