分枝PEI修饰的PLGA纳米粒转染DNA的研究.docVIP

分枝PEI修饰的PLGA纳米粒转染DNA的研究 季明辉胡贵方顾大勇；；；聚乳酸-羟基乙酸）聚乳酸-羟基乙酸）聚乳酸-羟基乙酸）中图分类号：文献标识码： 文章编号：Surface modification of PLGA nanoparticles with Branches polyethyleneimine for DNA transfection research JI Ming-hui1,2 , HU Gui-fang1△, GU Da-yong 2△，XIE Weidong3， TAN Shu-qin1，XU Hua4，OU Qin-ye4 (1 School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 515000,Guangdong Province, China; 2 Institute of Disease Control and Prevention, Shenzhen International Travel Health Care Center, Shenzhen Entry-exit Inspection and Quarantine Bureau, Shenzhen 518045, China ; 3 Graduate School at Shenzhen, Tsinghua University, Shenzhen 518000 Guangdong Province, China ; 4 School of Public Health, Nantong University, Nantong 226000，Jiangsu Province , China) ABSTRACT: Objective The aim of this study is to build up two PLGA nanoparticle (NP)-based gene delivery systems namely, plasmid DNA (pDNA) encapsulated PLGA NPs (PLGA-E) and surface adsorbed pDNA on PLGA-BPEI NPs (PLGA-BPEI), with respect to the extent of internalization and intracellular release of pDNA. Methods Several formulations have also been evaluated systematically for determination of the optimal transfection efficiency. The zeta-potential, particle size measurements and DNase I protection assay established the importance of the BPEI chain length in regulating the effective loading and condensation of pDNA with PLGA-BPEI NPs and pDNA protection ability of PLGA-BPEI NPs. The stability of these formulations was also investigated as a function of serum concentration. In vitro time-dependent gene transfection efficiencies were studied in presence as well as in absence of serum for NIH3T3 and HEK293 cells. The cell viability were also investigated using MTTassay. Results we build up two PLGA nanoparticle (NP)-based gene delivery systems namely, plasmid DNA (pDNA) encapsulated PLGA NPs (PLGA-E) and surface adsorbed pDNA on PLGA-BPEI NPs (PLGA-BPEI), the size of PLGA-E and PLGA-BPEI are between 200 to 270 nm，z