延胡索活性部位入血成分的LC-MSMS研究.pdfVIP

药学学报 Acta Pharmaceutica Sinica 2009, 44 (2): 167− 174 ·167 · 延胡索活性部位入血成分的LC-MS/MS 研究 * 程星烨, 石 钺 , 孙 虹, 金 文, 郑顺亮, 李开通, 杨 帅 ( 中国医学科学院、北京协和医学院药用植物研究所, 北京 100193) 摘要: 为深入研究延胡索活性部位体内作用的物质基础, 本文利用 LC-MS/MS 提供的色谱及离子碎片信息, 对口服延胡索活性部位后大鼠血浆中的移行成分进行分析鉴定, 通过保留时间比对、离子碎片解析等综合分析, 鉴定了 9 个入血成分均为原形成分, 同时也发现部分生物碱是以葡糖醛酸结合物的形式存在。入血成分及代谢 产物可能为延胡索体内直接作用的药效成分, 延胡索活性部位血清药物化学表征为阐明延胡索的药理作用及其 机制提供了依据。 关键词: 延胡索; 活性部位; 血清药物化学; 液相色谱-质谱 中图分类号: R917 文献标识码: A 文章编号: 0513-4870 (2009) 02-0167-08 Identification and analysis of absorbed components in rat plasma after oral administration of active fraction of Corydalis yanhusuo by LC-MS/MS * CHENG Xing-ye, SHI Yue , SUN Hong, JIN Wen, ZHENG Shun-liang, LI Kai-tong, YANG Shuai (Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China) Abstract: To analyze and identify the constituents in rat plasma after oral administration of the active fraction of Corydalis yanhusuo, a LC-MS/MS method was established. The constituents absorbed into blood, their original crude drugs and their metabolites were identified either by comparing the retention time and mass spectrometry data with that of reference compounds or by mass spectrometry analysis and retrieving the reference literatures. Nine species are the original form in Corydalis yanhusuo, moreover, some metabolites in blood identified as glucuronide were found. The constituents absorbed into blood and the possible metabolites whic