异丙酚对大鼠肝缺血再灌注时NF-κB活化的影响.docVIP

丙泊酚对大鼠肝再灌注损伤及NF-κB表达的作用 何静、王淑琼、陶国才(第三军医大学西南医院麻醉科，重庆 400038) [摘要] 目的 探讨丙泊酚对保护大鼠肝脏缺血/再灌注损伤（ischemia-reperfusion injury））(P＜0.0)，光镜下见肝细胞水肿、脂肪变性，并有点灶性坏死，肝血窦轻微淤血，NF-κB转录显著升高(P＜0.0)，肝组织总NF-κB蛋白量显著升高(P＜0.0)。应用丙泊酚后，肝ALT、AST酶升高受到显著抑制(P＜0.0)，肝细胞变性、坏死及肝血窦淤血减轻，肝组织总NF-κB蛋白表达量的升高程度却受到显著抑制(P＜0.0)，但NF-κB mRNA转录却进一步显著升高(P＜0.0)。结论 丙泊酚可减轻大鼠肝缺血/再灌注时损伤的程度并抑制NF-κB蛋白表达，NF-κB蛋白表达抑制可能是这种保护作用的分子机制之一。 [关键词] 丙泊酚、NF-κB、缺血/再灌注损伤、肝、大鼠 Propofol protects rat liver ischemia-reperfusion injury through the inhibition of NF-κB He Jing,Wang Shu-Qiong，TAO Guo-cai.Anesthesia department of Southwest Hospital, The Third Military Medical University, Chongqing 400038, China 【Abstract】 Objective To explore the role of nuclear factor-κB(NF-κB) during the protection of propofol on liver ischemia reperfusion(IR) injury. Methods 40 male rats were randomly divided into 4 equal groups: sham operation(N) group undergoing sham operation; ischemia reperfusion(IR) group undergoing partial hepatic ischemia and reperfusion .The left right and median lobe of the liver were subjected to ischemia for 1h and followed by reperfusion for 2h; propofol(P) group undergoing sham operation and perfusion 10 mg?kg-1?h-1 of propofol; propofol treatment(PIR) group undergoing ischemia reperfusion injury and perfusion propofol. RT-PCR was used to detect the transcription of NF-κB; Western blot was used to detect the expression of NF-κB in the liver. Result Compared with either the N or the P group, the IR group had obvious parenchymatous, fatty degeneration and punctiform necrosis in hepatocytes and lightly congestion in hepatic sinusoid, the ALT AST in the plasma in the IR group was significantly higher, and the expression value and the transcription value of NF-κB was significantly higher. The PIR group had less serious histopathologic change than the IR group, the increasing of ALT AST was significantly inhibited, the expression of NF-κB was significantly inhibited also, but the transcription of it was