冰草属(AgropyronGaertn)植物遗传多样性取样策略基于醇溶蛋白的研究.pdfVIP

2004,5(3) : 216~ 221 Journal of Plant Genetic Resources 冰草属(Agropy ron Gaertn.)植物遗传多样性取样 策略基于醇溶蛋白的研究 1, 2 1 2 车永和 , 李立会 , 何蓓如 1 2 ( , 100081; , 712100) : 对冰草属(A g rop y ron Gaertn. )植物的5 个种22 个居群分8 个取样梯度,分别随机取3691215182124 粒种 子的混 样进行醇溶蛋白的APAGE 分析, 结果发现: 平均Simpson 指数依取样量的增加表现增大, 以3 粒种子0922 表现最 低, 以21粒0933 表现最高,变异系数( V%) 为041醇溶蛋白谱带数依所提取的混 样的籽粒数目发生显著变化, 冰草属 内5 个种表现趋势相同,22 个居群不同取样整体平均以3 粒种子提取的醇溶蛋白谱带数 1838 为最少, 以 18 粒种子提取的 醇溶蛋白谱带2824 为最多, V% 为1321取样量达到 12 粒以上醇溶蛋白谱带差异不明显( P= 001) , 图谱表现基本一致 建议在利用生化指纹进行冰草属居群间及种间的遗传多样性研究中, 混 取样量最低应保持在 12 个个体及以上方能代表居 群整体,反映居群的整体遗传特性 : 冰草属; 居群; 醇溶蛋白; 取样;APAGE Sampling Strategy for Genetic Diversity in Agropyron Gaertn. Based on Gliadin 1, 2 1 2 HE Yonghe , LI Lihui , HE Beiru ( 1 I nst it ute of Crop Ger mp las m R esour ces , Ch inese A cademy of A g r ic utur al Sciences , Beij ing 100081 2 Colllege of A g rono my , N orthw est Sc iTech Un iv er sity of A gr icult ur e and For estry , Yangling Shaanx i 712 100 ) Abstract: An improved ph32 APAGE method was utilized to analyze the gliadin banding pattern with eight sampling methods 22 populations of 5 species in A g rop yron Gaertn. T he results showed that simpson in dexes increased along with the seed number in the bulked seed samples increasing. The whole gliadin banding patterns obviously changed among different seed samples. On the whole average, the most gliadin number was 2824 when seed number were 18, and the least was 1838 when bulked seed samples were 3 seeds. V% of the gliadin number was 1321 meanwhile, the gliadi