大鼠臂旁核内5-羟色胺阳性纤维和终末的来源.pdfVIP

Nov． 2009 25(6) 607～610 神 经 解 剖 学 杂 志 (ChineseJournalofNeuroanatomy) 大鼠臂旁核 内5一羟色胺阳性纤维和终末的来源① 吴利平 张 华 李 辉 李云庆 (第 四军医大学 西京医院惠宾病房 。基础部解剖学教研室，西安 710032) 摘 要 本研究采用荧光金(FG)逆行追踪与5一羟色胺 (5-HT)免疫荧光组化染色相结合的双重技术观察了臂旁核 (PBN)内5一 HT阳性神经纤维和终末的来源。将 FG注入PBN后，FG逆标神经元主要分布在三叉神经核簇、脑干网状结构外侧小细胞部、中 缝核簇和中脑导水管周 围灰质 (PAG)；免疫荧光组化染色的结果显示5-HT阳性神经元主要位于中缝核簇和PAG；在中缝核簇和 PAG内可见FG逆标并呈5-HT阳性的双重标记神经元。上述结果表明，中缝核簇和PAG内的5-HT能神经元向PBN发出投射， 它们在躯体和内脏感觉信息的传递和调控方面发挥重要作用。 关键词 5-羟色胺 臂旁核 中缝核簇 感觉信息 大鼠 Originsofserotonin—immunopositivefiberandterminalsin theparabrachial nucleusoftherat WuLiping ，ZhangHua ，LiHui，LiYunqing (HuiBinWard，XijingHospital；DepartmentofAnatomy，SchoolofBasicMedicine；theFourthMilitaryMedicalUniversity，Xian710032) Abstract EmployingFluoro—Gold(FG)retrogradetracingcombinedwithimmunofluoresencehistochemicalstainingforserotonin(5-HT) double—labelingtechnique，weinvestigatedthecentraloriginsofserotonin—immunopositivefibersandterminalsintheparabrachialnucleus (PBN)oftherat．AfterinjectingFGintotheunilateralPBN，FG-labeledneuronalcellbodiesweremainlyobservedinthetrigeminalsen— sorynuclei，parvocellularpartofthelateralreticularformationofthebrainstem，raphenucleiandmidbrainperiaqueductalrgay(PAG)． histochemicalstainingrevealedthat5-HT—immunopositiveneuronalcellbodiesweremainlyfoundinthe raphenucleiandPAG．WithintheraphenucleiandPAG，someof theFG—labeledneuronalcellbodieswerealsoshown5一HT—immunoposi— tivestaining．ThepresentresultssuggestthatserotoninergicneuronsintheraphenucleiandPAGprojeettothePBN．Theseserotoninergic neuronsmightplayimportantrolesinthetransmission，regulationandcontrolonbothsoamticandvisceralinformationprocessing． Keywords serotonin，parabrachial