丹参中苯丙氨酸裂解酶基因的克隆、诱导及其对酚酸类成分的影响.pdfVIP

Availableonlineatwww．sciencedirect．corn “稚 毒 Chinese 壤； ScienceDirect Journalof Natural ChineseJournalofNaturalMedicines2009，7(6)：0449--0457 M edicines doi：10．3724／SRJ．1009．2009．00449 一 CloningandInductionofPhenylalanineAmmonia-lyase Genefrom SalviamiltiorrhizaandItsEffectonHydrophilic PhenolicAcidsLevels HU Yong—Sheng ，ZHANG Lei，DIPeng ，CHEN Wan—Sheng DepartmentofPharmacy,ChangzhengHospital,SecondMilitaryMedicalUniversity,Shanghai200003； DepartmentofPharmacognosy,SchoolofPharmacy,SecondMilitaryMedical 1ersiO,,Shanghai200433 DepartmentofPharmacy,thell8thHospitalofPeA，Wenzhou325000,China Availableonline20Nov．2009 A【BSTRACT】AIM：ToClonephenylalanineammonia—lyasegene(designatedasSmpa1)from Salviamiltiorrhizaandtostudyits expression，inductionandeffectonhydrophilicphenolicacidslevelsinS．miltiorrhiza．METHoDS：Smpafwasclonedandwellchar— acterizedfrom mihiorrhiza．usingRapidAmplificationofcDNAends(RACE}technology．TheexpressionofSmpalcouldbein— ducedbvM eJA，SA andGA3．ThemetabolitecontentwasdeterminedbvLC MS．RESUIrS：Thefull—lengthofSmpalcDNAwas 2354bpinsizeandcontainedanORFof2133bpencoding711amino—acids．a5untranslatedregion(UTR1of21bp．anda3UTRof l71bp．RT．PCR analysisrevealedthattheSmpafexpressedconstitutivelyinaUtestplantorgaDSwiththehighestexpressionlevelin root．followedbystem andleaves．CONCLUSIoN：ThededucedSmpalsharedanoveral1identityof62．4％一93％withotherknown pals．Southem hybridizationanalysessuggestedthatSmpalbelongedtoamultigenefamily．Thef