斑马鱼CYP1A在DLCs毒理机制中的研究.pdfVIP

斑马鱼CYP1A在DLCs 毒理研究中的应用概况 1 2 1* 1 1 1 刘连平 ，聂芳红 ，陈进军 ，林红英 ，杨蓉 ，谢英明 （1 广东海洋大学农业生物技术研究所；2 广东海洋大学食品科技学院 广东湛江，524088 ） 摘 要：二噁英类化合物是一类广泛存在、具有广谱毒性的强毒性环境污染物。作为一种理想的脊椎动物模型，斑马鱼 已被应用于某些化学物的毒理学研究。本文综述了二噁英类化合物的理化特性、毒理作用和斑马鱼的生物学特性及其 CYP1A在二噁英类化合物毒理学研究中的研究新进展，指出可以利用斑马鱼独特的生物学特性，通过研究二噁英类化合 物对斑马鱼CYP1A的影响来揭示二噁英类化合物的生化毒理机制。 关键词：二噁英类化合物；毒理；CYP1A ；斑马鱼 Application of Zebrafish CYP1A into Toxicological Studies on Dioxin-like Compounds 1 2 1* 1 1 1 LIU Lian-ping ,NIE Fang-hong ,CHEN Jin-jun ,LIN Hong-ying ,YANG Rong ,XIE Ying-ming (1.Institute of Agricultural Biotechnology;2.Food Sci-Tech College,Guangdong Ocean University,Zhanjiang,Guangdong,524088,China) Abstract: Dioxin-like compounds (DLCs) are chemicals with ubiquity, broad-spectrum toxicity and highly toxic environmental pollution. Zebrafish is an ideal model animal of vertebrate and has been applied into toxicological researches of certain chemicals. The present paper reviewed certain new research progresses of DLCs in physical and chemical characteristics, toxicological effects and the biological property of zebrafish as well as the utilization of zebrafish CYP 1A in toxicological researches on DLCs. It was pointed out that the toxicological mechanism of DLCs can be explored by studies on the relationship between DLCs and zebrafish CYP1A because of the particular biological property of zebrafish. Key words: Dioxin-like compounds; toxicology; CYP1A; zebrafish 二噁英类化合物 （Dioxin-like compounds ，DLCs ）是指那些能与芳香烃受体（aryl hydrocarbon receptor ，AhR ）结合，并导致各种生物化学变化的化学物的总称，主要包括：多氯/溴代二苯并二噁英， 多氯/溴代二苯并呋喃和共平面多氯/溴联苯，其中研究最多也是最典型和毒性最强的物质为2,3,7,8- 四氯 代二苯并二噁英 (2,3,7,8-Tetrachlorodibenzo-p-dioxin，TCD