半纤维素制备方法的改善及其应用.pdfVIP

第 22 卷第 6 期 无 锡 轻 工 大 学 学 报 Vol. 22 　No. 6 　 　 　 　　　　 　 2003 年 11 月 Journal of Wuxi University of Light Industry 　 　 　　　　 Nov. 　2003 ( ) 文章编号 :1009038X 2003 　 半纤维素制备方法的改进及其应用 王雪鹏 , 　管 斌 , 　汤海青 , 　郭姗娜 ( 中国海洋大学 水产学院 ,山东 青岛 266003) 摘　要 : 对玉米芯中半纤维素的提取方法及条件进行改进 ,将蒸煮法与碱法提取相结合有效提高 了半纤维素的提取率和提取纯度. 以提取的半纤维素作为选择性碳源,可用来制备分离筛选木聚 糖酶产生菌的分离培养基 ; 探讨了半纤维素添加方式及添加量对培养基产透明圈效果的影响 . 关键词 : 木聚糖酶 ;半纤维素 ;分离培养基 中图分类号:Q 539 文献标识码 : A Improved Extraction Method for Hemicellulose and Its Application WAN G Xuepeng , 　GUAN Bin , 　TAN G Haiqing , 　GUO Shanna (College of Fisheries , Ocean University of China , Qingdao 266003 ,China) Abstract : In this paper , the extracting methods and conditions of hemicellulose from corncob were improved, the ratio and purit y of extraction were effectively raised by integration of cooking with alkalitreatment . The extracted hemicellulose were used as selective carbon source for selective medium to separate and screen xylanaseproducing strains. The addition manner and dose of hemicellulose which influenced transparent zones caused by the strains on the plate were studied. This selective medium could produce obvious transparent zones and the xylanase activity could be identified by these transparent zones elementarily. In this way , the efficiency of primary screening were effectively improved. Key words : xylanas ; hemicellulose ; selective medium 　　低聚木糖可有效促进体内双歧杆菌增殖 ,改善 培养基成分的质量直接关系到筛选效果. 由于纯木 肠道微生态环境 ,增强机体免疫力 ,并且与其它功 聚糖产品价格较高 , 以自制半纤维素作为选择性碳 能性低聚糖相比具有有效剂量低、酸热稳定性好、