固相微萃取技术收集水稻挥发物的比较.pdfVIP

中国农业科学 2012,45(4):809-814 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2012.04.023 固相微萃取技术收集水稻挥发物的比较 苏贻娟，王瑞龙，叶 茂，宋圆圆，曾任森 （华南农业大学热带亚热带生态研究所/农业部生态农业重点开放实验室，广州 510642 ） 摘要：【目的】探讨用固相微萃取技术（SPME）不同萃取头萃取到的水稻诱导挥发物的异同，为研究植物挥 发物提供方法依据。【方法】用茉莉酸甲酯处理水稻，分别用3 种萃取头：100 µm 聚二甲基硅氧烷（PDMS）、7 µm PDMS 和85 µm 聚丙烯酸酯（PA）萃取水稻的挥发物。用GC-MS 和GC 对挥发物进行定性定量分析，比较3 种萃取 头在萃取水稻挥发物的总量、成份和组成方面的异同，并与动态顶空收集法（DHS）收集的挥发物成份相比较。【结 果】在同样条件下，100 µm PDMS 萃取的挥发物总量和种类最多，7 µm PDMS 最少。SPME 法与DHS 法获得的挥发 物组成类别相似，但有些组分不同。【结论】不同萃取头对水稻挥发物的萃取效率和选择性是不同的。用SPME 法 研究水稻等植物挥发物，需根据目标组分的性质选择合适的萃取头。SPME 法与DHS 法在植物挥发物的收集和分析 中各有优缺点。 关键词：水稻挥发物；固相微萃取；动态顶空收集法 Comparison of Profiling Rice Volatiles by Means of Solid Phase Microextraction SU Yi-juan , WANG Rui-long, YE Mao, SONG Yuan-yuan, ZENG Ren-sen (Institute of Tropical Subtropical Ecology, South China Agricultural University/Key Laboratory of Ecological Agriculture, Ministry of Agriculture, Guangzhou 510642) Abstract ： 【Objective 】The objective of the study is to provide appropriate volatile collection methods of solid phase microextraction (SPME) for analyzing induced volatiles from plants through comparison of difference in rice volatile profiles collected by different fibres. 【Method 】 Three SPME fibres including 100 µm polydimethylsiloxane (PDMS), 7 µm PDMS and 85 µm polyacrylate （PA) were used to extract volatiles emitted from rice plants that were treated with methyl jasmonate. Gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) were used to compare the difference in rice induced volatiles in terms of their amount, profile and composition of extracted vol